文献类型：期刊文献

英文题名：Lactic acid bacteria, Enterococcus faecalis Y17 and Pediococcus pentosaceus G11, improved growth performance, and immunity of mud crab (Scylla paramamosain)

作者：Yang, Qiuhua[1,2];Lu, Yongling[1,2];Zhang, Ming[1,2];Gong, Yi[1,2];Li, Zhongzhen[1,2];Ngoc Tuan Tran[1,2];He, Yuyong[1,2];Zhu, Chunhua[3];Lu, Yishan[3];Zhang, Yueling[1,2];Li, Shengkang[1,2]

机构：[1]Shantou Univ, Guangdong Prov Key Lab Marine Biol, Shantou 515063, Peoples R China;[2]Shantou Univ, STU UMT Joint Shellfish Res Lab, Shantou 515063, Peoples R China;[3]Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Peoples R China

年份：2019

卷号：93

起止页码：135

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000488998300016)、、WOS

基金：The work was supported by grants from National Natural Science Foundation of China (41876152, 31850410487, 31802341), Guangdong provincial project of Science and Technology (2017B020204003), Department of Education of Guangdong Province (2017KCXTD014), the foundation of Guangdong provincial key laboratory of marine biotechnology (GPKLMB201803), support from Department of Ocean and Fisheries of Fujian Province (2017HYJG10) and Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams.

语种：英文

外文关键词：Mud crab; Probiotics; Vibrio parahaemolyticus; Enterococcus faecalis Y17; Pediococcus pentosaceus G11

外文摘要：Mud crabs (Scylla paramamosain), a commercially important cultured species in the southeastern region of China, is usually infected by Vibriosis or parasites, causing great economic losses in cultured farms. Previous studies have demonstrated that probiotics benefited in enhancing the immune response against invading pathogens in aquatic animals. In this study, the effects of dietary administration of lactic acid bacteria (LAB) (Enterococcus faecalis Y17 and Pediococcus pentosaceus G11) on growth performance and immune responses of mud crab were assessed. Both strains (Y17 and G11) showed an inhibitory activity against bacterial pathogens (Aeromonas hydrophila, Vibrio parahaemolyticus, Vibrio alginolyticus, Staphylococcus aureus, and beta Streptococcus), and a wide pH tolerance range of 2-10. In vivo, mud crabs were fed a control diet and experimental diets supplemented with 10(9) cfu g(-1) diet either Y17 or G11 for 6 weeks before subjecting to a challenge test with V. parahaemolyticus for 12 h. The probiotic-supplemented diets had significant effects on weight gain and specific growth rate during the feeding trial. Increased serum enzyme activities of phenoloxidase, lysozyme, and SOD were observed in the hemolymph of mud crab in Y17 and G11-supplemented groups compared to that in the controls (P < 0.01). The significantly up-regulated expression of gene CAT, LYS, proPO, and SOD could be seen in hepatopancreas in G11-supplemented groups. After the pathogenicity test, the survival rate of Y17 + and G11 + V. parahaemolyticus groups was 66.67% and 80.00%, respectively, compared with 53.33% for the control groups. Taken together, dietary supplementation of Y17 and G11 strains were beneficial in mud crab, which could increase growth performance, modulate immune system and protect the host against V. parahaemolyticus infection.