文献类型：期刊文献

英文题名：Genetic enhancement of Fujian oyster (Crassostrea angulata): Accelerated progeny growth through CRISPR/Cas9-mediated Myostatin-knockout

作者：Duan, Xixi[1,2,3,5];Zhang, Tao[1,3,5];Wan, Weitao[1,3,4,5,6];Huang, Jingyue[1,2,3,5];Zhao, Zhen[1,2,3,5];Wang, Shanshan[1,2,3,5];Wei, Zonglu[1,2,3,5];Li, Jun[1,3,4,5,6];Ma, Haitao[1,3,4,5,6];Yu, Ziniu[1,3,4,5,6];Qin, Yanping[1,3,4,5,6];Zhang, Yuehuan[1,3,4,5,6]

机构：[1]Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Key Lab Trop Marine Bioresources & Ecol, South China Sea Inst Oceanol,Guangdong Prov Key La, Guangzhou 510301, Peoples R China;[2]Guangxi Univ, Coll Anim Sci & Technol, Key Lab Aquat Hlth Breeding & Nutr Regulat Guangxi, Nanning 530004, Peoples R China;[3]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518120, Peoples R China;[4]Agrotech Extens Ctr Guangdong Prov, Guangzhou 510145, Peoples R China;[5]Chinese Acad Sci, Daya Bay Marine Biol Res Stn, Shenzhen 518124, Peoples R China;[6]Chinese Acad Sci, Marine Ecoenvironm Engn Res Inst, Hainan Prov Key Lab Trop Marine Biol Technol, Trop Marine Biol Res Stn Hainan, Sanya 572024, Peoples R China

年份：2026

卷号：48

外文期刊名：AQUACULTURE REPORTS

收录：SCI-EXPANDED(收录号：WOS:001779028500001)、、Scopus(收录号：2-s2.0-105038960830)、WOS

基金：This research was supported by National Key Research and Development Program of China (2022YFD2400305) ; Research on breeding technology of candidate species for Guangdong modern marine ranching (2024-MRB-00-001) ; Shellfish & Algae Industry Innovation Team of Guangdong Modern Agricultural Technology System (Grant no. 2024CXTD23) ; Shenzhen Basic Research Project (JCYJ20240813111707010) ; Guangdong Science and Technology Plan Programme (2024B1212050006) ; Hainan Provincial Key R & D Programme (ZDYF2024XDNY175; ZDYF2025XDNY088) ; Guangzhou Science and Technology Project (202206010133, 2023B03J00165; 2024B03J1226) ; Guangdong Basic and Applied Basic Research Foundation (2023A1515010944; 2022A1515010203) ; National Marine Genetic Resource Center; the earmarked fund for CARS-49; Guangdong oyster advantageous and characteristic industrial cluster project; and the Science and Technology Planning Project of Guangdong Province, China (2023B1212060047) .

语种：英文

外文关键词：Oyster; Gene editing; Edited populations; Performance traits; Genetic improvement

外文摘要：The growing global demand for aquatic protein underscores the importance of sustainable aquaculture. The Fujian oyster (Crassostrea angulata) is a key aquaculture species, yet its market value is constrained by its small size. Gene editing is a good tool for genetic improvement, while Myostatin (MSTN) gene has shown a great potential for enhancing growth in aquatic animals, we initiated a MSTN gene editing program in C. angulata. We designed and validated an sgRNA, constructed a CRISPR/Cas9 plasmid, and obtained a best electroporation condition. We then evaluated edited populations through to adulthood. The results showed that oyster gene editing can be completed by the transfection method. Mutant progeny have a normal growth, development, and survival. The mutant progeny has a better growth trait and larger adductor muscle, and their yearling progeny wet weight being over 53.10% greater than that of control group. Interestingly, we observed that mosaicism exhibited dynamic changes during the ontogeny of oysters. In conclusion, we successfully obtained mutant mosaic oyster adult lines for the first time, which provide a source for creating homozygous edited populations. This study establishes a foundation and demonstrates the potential for gene editing-based breeding in shellfish.