文献类型：期刊文献

英文题名：Activation of the endogenous acid protease in the head of Litopenaeus vannamei induced by UV-C irradiation

作者：Dou, Bijing[1]; Wang, He[1]; Cao, Wenhong[1,2,3,4,5]; Chen, Zhongqin[1,2,3,4]; Zhu, Guoping[1,2,3,4]; Zhang, Chaohua[1,2,3,4,5]; Zheng, Huina[1,2,3,4,5]; Lin, Haisheng[1,2,3,4,5]; Gao, Jialong[1,2,3,4,5]

机构：[1] College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 524088, China; [2] National Research and Development Branch Centre for Shellfish Processing [Zhanjiang], Zhanjiang, 524088, China; [3] Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Provincial Engineering Technology Research Centre of Seafood, Zhanjiang, 524088, China; [4] Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang, 524088, China; [5] Collaborative Innovation Centre of Seafood Deep Processing, Dalian Polytechnic University, Dalian, 116034, China

年份：2023

卷号：58

期号：3

起止页码：1401

外文期刊名：International Journal of Food Science and Technology

收录：EI(收录号：20230313403693)、Scopus(收录号：2-s2.0-85146362605)

语种：英文

外文关键词：Activation analysis - Amino acids - Chemical activation - Circular dichroism spectroscopy - Dichroism - Enzyme activity - Fluorescence spectroscopy - Fourier transform infrared spectroscopy - Hydrogen bonds - Microwave irradiation - Sodium chloride

外文摘要：Previous studies have found that UV-C irradiation activated endogenous acid protease (EAP) in shrimp heads. Aiming at understanding the effect of UV-C irradiation on the activation of EAP and improving the utilisation of EAP, the effect of UV-C irradiation on the enzymatic properties and conformation of EAP was investigated. The enzymatic properties of EAP were unaltered after UV-C irradiation, and the optimum pH, temperature, and NaCl concentration were 3, 60°C, and 0.5 mol/L, respectively. Ca2+ and Mg2+ activated the enzyme. The molecular weight of the purified endogenous acid protease (P-EAP) was 32 kDa. UV–Vis, FTIR, circular dichroism, and fluorescence spectroscopy indicated that the increase in enzyme activity by UV-C irradiation was related to an increase in tryptophan and hydrogen bonding, implying that it may be the change in the spatial structure of the protein that caused the activation of the enzyme. Results revealed that UV-C irradiation can be used to activate proteases and provide a reference for enhancing the enzymatic activity of other enzymes from shrimp heads. ? 2023 Institute of Food, Science and Technology (IFSTTF).