文献类型：期刊文献

英文题名：Detection of AFB₁ via TiO₂ Nanotubes/Au Nanoparticles/Enzyme Photoelectrochemical Biosensor

作者：Yuan, Qiong[1];He, Chuxian[1];Mo, Rijian[1];He, Lei[1];Zhou, Chunxia[1,2,3];Hong, Pengzhi[1,2,3];Sun, Shengli[4,5,6];Li, Chengyong[1,2,3,4,5]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518108, Peoples R China;[3]Guangdong Ocean Univ, Guangdong Modern Agr Sci & Technol Innovat Ctr, Zhanjiang 524088, Peoples R China;[4]Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Peoples R China;[5]Guangdong Ocean Univ, Coastal Ecol Engn Technol Res Ctr Zhanjiang City, Zhanjiang 524088, Peoples R China;[6]Guangdong Ocean Univ, Ctr Anal & Test, Zhanjiang 524088, Peoples R China

年份：2018

卷号：8

期号：3

外文期刊名：COATINGS

收录：SCI-EXPANDED(收录号：WOS:000428506500006)、、WOS

基金：This work is supported by Science and Technology Planning Project of Guangdong Province (2016A020210114), Science and Technology Planning Project of Zhanjiang City (2015A03025, 2016C01002), Industrial Development Special Funds of Dapeng New Area (KY20170209), Training Programs of Innovation and Entrepreneurship for Undergraduates (201510566003, 524000087267, CXXL2015003, CXXL2015087, CXXL2015088, CXXL2015089).

语种：英文

外文关键词：photoelectrochemical; aflatoxin B-1; acetylcholinesterase; TiO2 nanotubes

外文摘要：TiO2 nanotubes/Au nanoparticles/enzyme photoelectrochemical biosensor is developed by the chemical bonding of acetylcholinesterase with Au nanoparticles-modified TiO2 photoactive electrode, based on the inhibitory effect of aflatoxin B-1 on acetylcholinesterase activity. In this method, AuNPs were deposited on the surface of the electrode by potentiostatic deposition and the acetylcholinesterase was chemically crosslinked to the surface for determination of aflatoxin B-1. Enzymatic hydrolysate is generated to capture the photogenerated holes of UV-sensitized TiO2 nanotube arrays, causing magnification of the photoelectrochemical signal. The photoelectrochemical biosensor morphological and structural details were evaluated, applying different techniques, such as X-ray Diffraction (XRD) and Scanning Electron Microscopy (SEM). Aflatoxin B-1 competitively inhibits acetylcholinesterase, leading to a decrease in photocurrent that should have been increased. The detection performance of biosensors for different concentrations of AFB(1) is discussed. The linear response range of the biosensor is from 1-6 nM with detection limitation of 0.33 nM, the linear equation is I (mu A) = -0.13C (nM) + 9.98 (mu A), with a correlation coefficient of 0.988. This new biosensor could be used to detect Aflatoxin B1 in foods.