文献类型：期刊文献

英文题名：Fucoidan from Laminaria japonica Inhibits Expression of GLUT9 and URAT1 via PI3K/Akt, JNK and NF-κB Pathways in Uric Acid-Exposed HK-2 Cells

作者：Zhang, Yu[1,2];Tan, Xiaohui[1,2];Lin, Zhen[1];Li, Fangping[1];Yang, Chunyan[2];Zheng, Haiying[2];Li, Lingyu[2];Liu, Huazhong[1];Shang, Jianghua[2]

机构：[1]Guangdong Ocean Univ, Coll Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[2]Chinese Acad Agr Sci, Guangxi Buffalo Res Inst, Guangxi Key Lab Buffalo Genet Reprod & Breeding, Nanning 530001, Peoples R China

年份：2021

卷号：19

期号：5

外文期刊名：MARINE DRUGS

收录：SCI-EXPANDED(收录号：WOS:000654427900001)、、Scopus(收录号：2-s2.0-85105218233)、WOS

基金：This work was supported by grants from the Natural Science Foundation of Guangdong Province (2019A1515011102), the Project of Application-Based Talent Training Course from Guangdong Ocean University (570319017, 571119165), the National Key Research and Development Program (2017YFE0113800), the Key Research and Development Program in Guangxi (GuiKe AB1850013), and the Guangxi Natural Science Foundation (2018GXNSFDA050013), China.

语种：英文

外文关键词：fucoidan; uric acid; urate transporter 1; glucose transporter 9

外文摘要：This work aimed to investigate the effect of fucoidan (FPS) on urate transporters induced by uric acid (UA). The results showed that UA stimulated the expression of glucose transporter 9 (GLUT9) and urate transporter 1 (URAT1) in HK-2 cells, and FPS could reverse the effect. Moreover, UA could activate NF-kappa B, JNK and PI3K/Akt pathways, but both pathway inhibitors and FPS inhibited the UA-induced activation of these three pathways. These data suggested that FPS effectively inhibited the expression induction of reabsorption transporters URAT1 and GLUT9 by UA, through repressing the activation of NF-kappa B, JNK and PI3K/Akt signal pathways in HK-2 cells. The in vitro research findings support the in vivo results that FPS reduces serum uric acid content in hyperuricemia mice and rats through inhibiting the expression of URAT1 and GLUT9 in renal tubular epithelial cells. This study provides a theoretical basis for the application of FPS in the treatment of hyperuricemia.