文献类型：期刊文献

英文题名：Glucose Oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing

作者：Kang, Xinhuang[1,2];Wang, Jun[1];Wu, Hong[1];Aksay, Ilhan A.[3];Liu, Jun[1];Lin, Yuehe[1]

机构：[1]Pacific NW Natl Lab, Richland, WA 99352 USA;[2]Guangdong Ocean Univ, Coll Sci, Zhanjiang 524088, Guangdong, Peoples R China;[3]Princeton Univ, Dept Chem Engn, Princeton, NJ 08544 USA

年份：2009

卷号：25

期号：4

起止页码：901

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：SCI-EXPANDED(收录号：WOS:000272439800039)、、EI(收录号：20094512417581)、Scopus(收录号：2-s2.0-70350394842)、WOS

基金：This work was supported by a laboratory-directed research and development program (LDRD) at Pacific Northwest National Laboratory (PNNL). The work was performed at the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the U.S. Department of Energy (DOE) and located at PNNL. PNNL is operated by Battelle for DOE under Contract DE-AC05-76RL01830. IAA acknowledges support from ARO/MURI under grant number W911NF-04-1-0170.

语种：英文

外文关键词：Graphene; Glucose oxidase; Direct electron transfer; Chitosan

外文摘要：Direct electrochemistry of a glucose oxidase (GOD)-graphene-chitosan nanocomposite was studied. The immobilized enzyme retains its bioactivity, exhibits a surface confined, reversible two-proton and two-electron transfer reaction, and has good stability, activity and a fast heterogeneous electron transfer rate with the rate constant (k(s)) of 2.83 s(-1). A much higher enzyme loading (1.12 x 10(-9) mol/cm(2)) is obtained as compared to the bare glass carbon surface. This GOD-graphene-chitosan nanocomposite film can be used for sensitive detection of glucose. The biosensor exhibits a wider linearity range from 0.08 mM to 12 mM glucose with a detection limit of 0.02 mM and much higher sensitivity (37.93 mu A mM(-1) cm(-2)) as compared with other nanostructured supports. The excellent performance of the biosensor is attributed to large surface-to-volume ratio and high conductivity of graphene, and good biocompatibility of chitosan, which enhances the enzyme absorption and promotes direct electron transfer between redox enzymes and the surface of electrodes. (C) 2009 Elsevier B.V. All rights reserved.