文献类型：期刊文献

英文题名：Identification and comprehensive evaluation of a novel biocontrol agent Bacillus atrophaeus JZB120050

作者：Ni, Mi[1]; Wu, Qiong[1]; Wang, Junli[2]; Liu, Wei C.[1]; Ren, Jian H.[3]; Zhang, Dian P.[1]; Zhao, Juan[1]; Liu, D.E.W.[1]; Rao, Ying H.[1]; Lu, Cai G.[1]

机构：[1] Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China; [2] Agricultural College, Guangdong Ocean University, Zhanjiang, China; [3] Suzhou BioNovoGene Metabolomics Platform, Suzhou, China

年份：2018

卷号：53

期号：12

起止页码：777

外文期刊名：Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes

收录：EI(收录号：20183805831331)

语种：英文

外文关键词：Hydrocarbons - Bacteriology - Escherichia coli - Gas chromatography - Mass spectrometry - Antimicrobial agents - Biocontrol - Cloning - Genes - Metabolites

外文摘要：Bacillus spp. have long been used as biocontrol agents because of their efficient broad-spectrum antimicrobial activity. We identified a novel strain of Bacillus atrophaeus, named JZB120050, from soil. B. atrophaeus JZB120050 had a strong inhibitory effect against Botrytis cinerea and many other phytopathogens. Gas chromatography-mass spectrometry showed that B. atrophaeus JZB120050 produced many secondary metabolites, such as alkanes, alkenes and acids; some of which were related to pathogen inhibition. Enzyme activity analysis showed that B. atrophaeus JZB120050 secreted cell-wall-degrading enzymes, including chitinase, glucanase and protease, which degraded fungal cell walls. Both the novel glucanase gene bglu and chitinase gene chit1 were cloned and heterologously expressed in Escherichia coli and the products showed strong enzyme activity. In addition, B. atrophaeus JZB120050 secreted siderophores and formed a significant biofilm. Future studies should focus on these antimicrobial factors to facilitate widespread application in the field of agricultural biocontrol. ? 2018, ? 2018 Taylor & Francis Group, LLC.