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狗舌草提取物诱导L1210细胞的凋亡     被引量:8

Apoptosis of L1210 Cell Line Induced by Extract from Tephroseris Kirilowii Turcz Holub in vitro

文献类型:期刊文献

中文题名:狗舌草提取物诱导L1210细胞的凋亡

英文题名:Apoptosis of L1210 Cell Line Induced by Extract from Tephroseris Kirilowii Turcz Holub in vitro

作者:陈进军[1];王建华[2]

机构:[1]广东海洋大学农学院动物医学系;[2]西北农林科技大学动物科技学院

年份:2006

卷号:37

期号:3

起止页码:295

中文期刊名:畜牧兽医学报

外文期刊名:Acta Veterinaria et Zootechnica Sinica

收录:CSTPCD、、北大核心2004、CSCD2011_2012、北大核心、CSCD

基金:教育部留学回国人员科研启动基金(教外司留[2003]406号);国家杨凌农业高新技术产业示范区科技专项(1999KG13)

语种:中文

中文关键词:狗舌草提取物;L1210细胞;流式细胞术;细胞凋亡

外文关键词:extract from Tephroseris kirilowii Turcz Holub;murine L1210 cell line;flow cytometry, apoptosis

中文摘要:以环磷酰胺、槲皮素和单猪屎豆碱为参照,观察狗舌草60%乙醇提取物体外对L1210细胞的凋亡形态变化的影响;利用流式细胞术AnnexinⅤ-FITC/PI双参数法,研究狗舌草60%乙醇提取物对L1210细胞早期凋亡的影响,探讨狗舌草60%乙醇提取物致L1210细胞凋亡的机理。结果发现,狗舌草60%乙醇提取物能使L1210细胞发生典型的细胞凋亡,形成凋亡小体;狗舌草60%乙醇提取物引起的AnnexinⅤ-FITC+/PI-细胞百分比为86.8%,能诱导L1210细胞发生早期凋亡。

外文摘要:With a reference system of cyclophosphamide (CY), quercetin (QU) and monocrotaline (MO), the apoptotic cellular morphology of murine L1210 induced by the extract from Tephroseris kirilowii Turcz Holub with 60% ethanol solution (EX) was observed in vitro. The early ap optotic actions of EX to murine L1210 cells were studied by Annexin V-FITC/PI of flow cytometry and the apoptotic mechanism of EX to murine L1210 was discussed. The results showed that EX at 1.0μg/mL made murine L1210 cells become apoptotic bodies in morphology and EX induced murine L1210 cells into early apoptosis with 86.8% Annexin V-FITC^+/PI^- cells.

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