文献类型：期刊文献

英文题名：Bta-miR-152 affects intracellular triglyceride content by targeting the UCP3 gene

作者：Shen, Binglei[1];Han, Shuo[1];Wang, Yuxuan[1];Yang, Zhuonina[1];Zou, Ziwen[1];Liu, Juan[1];Zhao, Zhihui[2];Wu, Rui[1];Wang, Changyuan[1]

机构：[1]Heilongjiang Bayi Agr Univ, Coll Anim Sci & Vet Med, Daqing 163319, Peoples R China;[2]Guangdong Ocean Univ, Agr Coll, Zhanjiang, Peoples R China

年份：2019

卷号：103

期号：5

起止页码：1365

外文期刊名：JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION

收录：SCI-EXPANDED(收录号：WOS:000478470300001)、、Scopus(收录号：2-s2.0-85072153629)、WOS

基金：National Natural Science Foundation of China, Grant/Award Number: 31472249 and 31772562; National Major Special Project on New Varieties Cultivation for Transgenis Organisms, Grant/Award Number: 2016ZX08009003-006; Heilongjiang Bayi Agricultural University Postdoctoral Startup Fund Project, Grant/Award Number: XDB-2017-06; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, Grant/Award Number: PCBD201708; Heilongjiang Province Postdoctoral Startup Fund Project, Grant/Award Number: LBH-Z16166; Heilongjiang Bayi Agricultural University Youth Innovation Talent Project, Grant/Award Number: CXRC-2016-06; Heilongjiang Provincial Department of Education Science and Technology Research Project, Grant/Award Number: 12521365; China Postdoctoral Science Foundation, Grant/Award Number: 2018M631970;

语种：英文

外文关键词：bta-miR-152; mammary epithelial cells; PRKAA1; triglyceride; uncoupling proteins 3

外文摘要：According to our previous studies, bta-miR-152, PRKAA1 and UCP3 are differentially expressed in mammary gland tissues of high milk fat and low milk fat cows, and the trend in bta-miR-152 expression is opposite from those of PRKAA1 and UCP3. To further identify the function and regulatory mechanism of bta-miR-152 in milk fat metabolism, we investigated the effect of bta-miR-152 on cellular triglyceride content in bovine mammary epithelial cells cultured in vitro, on the basis of bta-miR-152 overexpression and inhibition assays. The target genes of bta-miR-152 were identified through qPCR, Western blotting and dual luciferase reporter gene detection. Compared with that in the control group, the expression of UCP3 was significantly lower in the bta-miR-152 mimic group, the expression of PRKAA1 was decreased, and the intracellular TAG content was significantly increased. After transfection with bta-miR-152 inhibitor, the expression of UCP3 increased significantly, and the expression of PRKAA1 decreased, but the difference was not significant; in addition, the intracellular TAG content decreased significantly. Therefore, we concluded that bta-miR-152 affects the intracellular TAG content by targeting UCP3.