文献类型：期刊文献

英文题名：Comparative Physiological and Transcriptomic Profiling Offers Insight into the Sexual Dimorphism of Hepatic Metabolism in Size-Dimorphic Spotted Scat (Scatophagus argus)

作者：Chen, Huapu[1];Jiang, Dongneng[1];Li, Zhiyuan[1];Wang, Yaorong[1];Yang, Xuewei[2];Li, Shuangfei[2];Li, Shuisheng[3,4];Yang, Wei[1,5];Li, Guangli[1]

机构：[1]Guangdong Ocean Univ, Key Lab Marine Ecol & Aquaculture Environm Zhanji, Coll Fisheries, Guangdong Res Ctr Reprod Control & Breeding Techn, Zhanjiang 524088, Peoples R China;[2]Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen 518052, Peoples R China;[3]Sun Yat Sen Univ, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China;[4]Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou 510275, Peoples R China;[5]Yangjiang Polytech, Food & Environm Engn Dept, Yangjiang 529566, Peoples R China

年份：2021

卷号：11

期号：6

外文期刊名：LIFE-BASEL

收录：SCI-EXPANDED(收录号：WOS:000665927900001)、、WOS

基金：This study was supported by grants from the National Key R&D Program of China (2018YFD0901203), Special key projects of Guangdong Science and Technology Department's science and technology innovation strategy (2018B030311050), Guangdong Basic and Applied Basic Research Foundation (2019A1515010958 and 2019A1515012042) and Guangdong South China Sea Key Laboratory of Aquaculture for Aquatic Economic Animals, Guangdong Ocean University (No. KFKT2019ZD07).

语种：英文

外文关键词：Scatophagus argus; sexual size dimorphism; liver; RNA-Seq; metabolism; enzyme activity

外文摘要：The spotted scat (Scatophagus argus) is an economically important cultured marine fish that exhibits a typical sexual size dimorphism (SSD). SSD has captivated considerable curiosity for farmed fish production; however, up till now the exact underlying mechanism remains largely unclear. As an important digestive and metabolic organ, the liver plays key roles in the regulation of fish growth. It is necessary to elucidate its significance as a downstream component of the hypothalamic-pituitary-liver axis in the formation of SSD. In this study, the liver physiological differences between the sexes were evaluated in S. argus, and the activity of several digestive and metabolic enzymes were affected by sex. Females had higher amylase, protease, and glucose-6-phosphate dehydrogenase activities, while males exhibited markedly higher hepatic lipase and antioxidant enzymes activities. A comparative transcriptomics was then performed to characterize the responsive genes. Illumina sequencing generated 272.6 million clean reads, which were assembled into 79,115 unigenes. A total of 259 differentially expressed genes were identified and a few growth-controlling genes such as igf1 and igfbp1 exhibited female-biased expression. Further analyses showed that several GO terms and pathways associated with metabolic process, particularly lipid and energy metabolisms, were significantly enriched. The male liver showed a more active mitochondrial energy metabolism, implicating an increased energy expenditure associated with reproduction. Collectively, the female-biased growth dimorphism of S. argus may be partially attributed to sexually dimorphic metabolism in the liver. These findings would facilitate further understanding of the nature of SSD in teleost fish.