文献类型：期刊文献

英文题名：Molecular cloning and characterisation of scavenger receptor class B in pearl oyster Pinctada fuctada martensii

作者：Lei, Chao[1]; Hao, Ruijuan[1]; Zheng, Zhe[1]; Deng, Yuewen[1,2]; Wang, Qingheng[1,2]; Li, Junhui[1]

机构：[1] Fisheries College, Guangdong Ocean University, Zhanjiang, 524088, China; [2] Pearl Breeding and Processing Engineering Technology Research Center of Guangdong Province, Zhanjiang, 524088, China

年份：2017

卷号：30

起止页码：12

外文期刊名：Electronic Journal of Biotechnology

收录：EI(收录号：20174704415506)、Scopus(收录号：2-s2.0-85034088263)

语种：英文

外文关键词：Amino acids - Shellfish - Cloning - Transcription - Proteins - Histology - Hydrophobicity - Polymerase chain reaction - Sulfur compounds - Tissue - Molluscs

外文摘要：Background Molluscs can accumulate carotenoids in their body tissues by predominantly feeding on aquatic plant sources. Carotenoid transport and absorption are determined by the regulation of various proteins such as Scavenger receptor class B(SR-BI). We report the identification and characterisation of pearl oyster Pinctada fuctada martensii SR-BI (PmSR-BI). The correlation between total carotenoid content (TCC) and gene expression was also estimated. Results The full-length cDNA of PmSR-BI was 1828 bp, including an open-reading frame encoding of 1518 bp with a pI value of 5.83. PmSR-BI protein contains a hydrophobic CD36 domain and four centrally clustered cysteine residues for the arrangement of disulphide bridges. The deduced amino acid sequence had an identity of 30% to 60% with the SR-B of other organisms. Reverse transcription polymerase chain reaction analysis showed that mRNA transcripts were expressed in multiple tissues of adult pearl oyster. A higher expression of PmSR-BI gene was observed in the hepatopancreas than in the adductor muscle, gill and mantle. The TCC and gene expression of PmSR-BI were significantly correlated (P ? 2017