文献类型：期刊文献

英文题名：Anti-Photoaging Effects of Nanocomposites of Amphiphilic Chitosan/18β-Glycyrrhetinic Acid

作者：Quan, Weiyan[1];Kong, Songzhi[2];Li, Sidong[2];Ouyang, Qianqian[1];Lu, Sitong[3];Guo, Jiaqi[2];Wu, Kefeng[1];Zhao, Wei[4];Luo, Hui[1]

机构：[1]Guangdong Med Univ, Marine Biomed Res Inst, Zhanjiang 524023, Peoples R China;[2]Guangdong Ocean Univ, Sch Chem & Environm Sci, Dept Appl Chem, Zhanjiang 524088, Peoples R China;[3]Songshan Lake Mat Lab, Dongguan 523808, Peoples R China;[4]Southern Med Univ, Sch Publ Hlth, Guangdong Prov Key Lab Trop Dis Res, Guangzhou 510515, Peoples R China

年份：2023

卷号：28

期号：11

外文期刊名：MOLECULES

收录：SCI-EXPANDED(收录号：WOS:001006091600001)、、EI(收录号：20230033640)、Scopus(收录号：2-s2.0-85161616798)、WOS

基金：This work was supported by the National Natural Science Foundation of China (81803684), the Discipline Construction Project of Guangdong Medical University (4SG22264G, 4SG22241G, 4SG22009G), and the Doctoral Start-up Fund of Guangdong Medical University (GDMUB2021005, GDMUB2022032).

语种：英文

外文关键词：amphiphilic chitosan; 18 beta-glycyrrhetinic acid; nanocrystals; hydrogel; skin photoaging

外文摘要：Improving the transdermal absorption of weakly soluble drugs for topical use can help to prevent and treat skin photoaging. Nanocrystals of 18 beta-glycyrrhetinic acid (i.e., NGAs) prepared by high-pressure homogenization and amphiphilic chitosan (ACS) were used to form ANGA composites by electrostatic adsorption, and the optimal ratio of NGA to ACS was 10:1. Dynamic light scattering analysis and zeta potential analysis were used to evaluate the nanocomposites' suspension, and the results showed that mean particle size was 318.8 +/- 5.4 nm and the zeta potential was 30.88 +/- 1.4 mV after autoclaving (121 degrees C, 30 min). The results of CCK-8 showed that the half-maximal inhibitory concentration (IC50) of ANGAs (71.9 mu g/mL) was higher than that of NGAs (51.6 mu g/mL), indicating that the cytotoxicity of ANGAs was weaker than that of NGAs at 24 h. After the composite had been prepared as a hydrogel, the vertical diffusion (Franz) cells were used to investigate skin permeability in vitro, and it was shown that the cumulative permeability of the ANGA hydrogel increased from 56.5 +/- 1.4% to 75.3 +/- 1.8%. The efficacy of the ANGA hydrogel against skin photoaging was studied by constructing a photoaging animal model under ultraviolet (UV) irradiation and staining. The ANGA hydrogel improved the photoaging characteristics of UV-induced mouse skin significantly, improved structural changes (e.g., breakage and clumping of collagen and elastic fibers in the dermis) significantly, and improved skin elasticity, while it inhibited the abnormal expression of matrix metalloproteinase (MMP)-1 and MMP-3 significantly, thereby reducing the damage caused by UV irradiation to the collagen-fiber structure. These results indicated that the NGAs could enhance the local penetration of GA into the skin and significantly improve the photoaging of mouse skin. The ANGA hydrogel could be used to counteract skin photoaging.