文献类型：期刊文献

英文题名：Molecular cloning, characterization and expression analysis of spexin in spotted scat (Scatophagus argus)

作者：Deng, Si-ping[1,2,3];Chen, Hua-pu[1,2];Zhai, Yi[1];Jia, Li-ying[1];Liu, Jian-ye[1,2];Wang, Mei[1,2];Jiang, Dong-neng[1,2];Wu, Tian-li[1,2];Zhu, Chun-hua[1,2];Li, Guang-li[1,2]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Guangdong Res Ctr Reprod Control & Breeding Techn, Zhanjiang 524088, Peoples R China;[3]Marine Ecol & Aquaculture Environm Zhanjiang, Zhanjiang 524088, Peoples R China

年份：2018

卷号：266

起止页码：60

外文期刊名：GENERAL AND COMPARATIVE ENDOCRINOLOGY

收录：SCI-EXPANDED(收录号：WOS:000442707700007)、、WOS

基金：This study was funded by Natural Science Foundation of Guangdong Province (2016A030313743, 2017A030313101), The National Natural Science Foundation of China (41706174, 31702326), Marine Fishery Science and Technology Extension Projects of Guangdong (A201608B01, 2017A0012), Zhanjiang Science and Technology Bureau (2016A03017) and the College Students' innovation and Entrepreneurship Project (201610566007, 201410566046).

语种：英文

外文关键词：Scatophagus argus; Spexin; Reproduction; 17 beta-Estradiol; Feeding control; Ovarian development stages

外文摘要：Spexin (Spx), a novel neuropeptide, composed of 14 amino acid residues, is evolutionally conserved from fish to mammals It has been suggested that Spx has pleiotropic functions in mammals. However, reports about Spx are very limited. To clarify the roles of Spx in the regulation of reproduction and food-intake in the spotted scat, the spx (ssspx) gene was cloned and analyzed. Analysis of the tissue distribution by RT-PCR showed that ssspx expression was widespread. During ovary development, expression of ssspx was found to be highest in phase II, moderate in phase III, and at its lowest level in phase IV. Ssspx expression was significantly down-regulated in the hypothalamus after treatment with E-2 both in vitro and in vivo. A significant increase of ssspx was observed after 2 and 7 days of food deprivation. However, the ssspx transcript levels in the 7 day fasting group decreased significantly after refeeding 3 h after the scheduled feeding time. This suggests that ssSpx may be involved in the regulation of reproduction and food-intake in the spotted scat.