文献类型：期刊文献

中文题名：米曲霉中性蛋白酶特性的研究

英文题名：Study on Characters of Neutral Protease from Aspergillus oryzae

作者：杨世平[1];邱德全[1]

机构：[1]广东海洋大学广东省海洋开发研究中心,广东湛江524025

年份：2006

卷号：26

期号：4

起止页码：22

中文期刊名：湛江海洋大学学报

外文期刊名：Journal of Zhanjiang Ocean University

收录：CSTPCD

基金：湛江市科技局招标项目(2002074)

语种：中文

中文关键词：米曲霉;中性蛋白酶;提取方法;酶活力

外文关键词：Aspergillus oryzae ; neutral protease; extration condition;enzyme activity

中文摘要：研究了米曲霉中性蛋白酶的特性。结果表明,米曲霉中性蛋白酶的最佳提取方法为加入pH 7.2的缓冲液研匀,置恒温(30℃)培养箱中提取30 min,每10 min搅动1次,最后用4层纱布过滤。酶反应最适温度为55℃,最适pH值为7.2。NaCl和EDTA对其有一定的抑制作用。酶的热稳定性好,在45℃的温度下处理24 h后,酶活力仍有45.5%。米曲霉中性蛋白酶与培养物一起保存时,稳定性也较好,在4℃保存一个月后,酶活力几乎无变化。

外文摘要：The characters of neutral protease from Aspergillus oryzae were studied. The results show that the suitable extraction conditions of neutral protease are under 30℃, pH 7. 2, 30 min. The extraction is stirred per 10 min, finally filtrated with four layers of gauze. The optimal pH and temperature for enzyme activity are 7.2 and 55℃. Sodium chloride（NaC1） and EDTA inhibit the enzyme. The enzyme is stable, which retains 45.5% of its original activity after being treated under 45℃ for 24 h. The enzyme is also stable while preserved with medium, for its activity is almost unchangeable after having been preserved under 4℃ for a month.