文献类型：期刊文献

中文题名：马氏珠母贝Pm-miR-183序列分析及功能研究

英文题名：Sequence Analysis and Function Study of Pm-miR-183 in Pinctada fucata martensii

作者：熊新威[1];郑哲[1];焦钰[1];邓岳文[1];杜晓东[1]

机构：[1]广东海洋大学水产学院广东省珍珠养殖与加工工程技术研究中心,广东湛江524088

年份：2017

卷号：37

期号：6

起止页码：11

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：CSTPCD

基金：国家自然科学基金(31672626);广东省海洋渔业科技推广专项(B201601-Z10)

语种：中文

中文关键词：马氏珠母贝;Pm-miR-183;生物矿化;miRNA调控

外文关键词：Pinctada fucata martensii;Pm-miR-183;biomineralization;miRNA regulation

中文摘要：用马氏珠母贝基因组序列,通过生物信息学方法获得Pm-miR-183的前体,长度76 bp,具有典型的茎环结构。成熟序列及前体序列的同源性分析均显示较高的种间保守性。Pm-miR-183前体序列(Pm-pre-miR-183)的系统进化树分析,Pm-pre-miR-183与帽贝同源性较高。靶向关系分析,Pm-miR-183与矿化基因及免疫相关基因均存在靶向作用位点。实时荧光定量分析,Pm-miR-183在马氏珠母贝的各组织中均有表达。注射Pm-miR-183mimics进行过表达,Pm-miR-183的表达量在套膜区(MP)中显著上调(P<0.05)。扫描电子显微镜(scanning electron microscope,SEM)检测,Pm-miR-183过表达会导致贝壳珍珠层生长出现紊乱。

外文摘要：MiRNAs are non-coding small RNAs involved in negatively transcriptional regulation. In our previous study, we had identified a miR-183 homologue in Pinctada fucata martensii by Solexa deep sequencing. Based on bioinformatics results obtained from whole genome sequence of P. f. martensii, the precursor Pm-miR-183（Pm-pre-miR-183）obtained has a length of 76 bp and a typical stem-loop structure. Homology analysis of mature sequence and previous sequence indicated that they were highly conserved with other species. Phylogenetic analysis of precursor sequence showed that Pm-pre-miR-183 had a higher homology to Lottia gigante. The potential interactions among Pm-miR-183 and several mineralized genes and immune-related genes were obtained by target prediction. Pm-miR-183 was expressed in different tissues. In addition, after injection of Pm-miR-183 mimics, the expression of Pm-miR-183 was significantly up-regulated in the mantle pallial. The result of SEM showed that there were adnormal growth of nacre in shell. This study shows that Pm-miR-183 could be involved in the formation of pearl oyster in P. f. martensii.