文献类型：期刊文献

英文题名：SMRT Sequencing of the Full-Length Transcriptome of the Coelomactra antiquata

作者：Deng, Aiping[1,2];Li, Jinpeng[1,2];Yao, Zebin[1,2];Afriyie, Gyamfua[1,2];Chen, Ziyang[1,2];Guo, Yusong[1,2];Luo, Jie[1,2];Wang, Zhongduo[1,2]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang, Peoples R China;[2]Guangdong Ocean Univ, Fisheries Coll, Guangdong Prov Key Lab Aquaculture South China Se, Zhanjiang, Peoples R China

年份：2021

卷号：12

外文期刊名：FRONTIERS IN GENETICS

收录：SCI-EXPANDED(收录号：WOS:000713903100001)、、Scopus(收录号：2-s2.0-85118293425)、WOS

基金：This work was supported by the project of Zhanjiang Agricultural and Rural Bureau under Zhancainong (2019) No. 152; Zhanjiang Municipal Bureau of Agriculture and Rural Affairs, the central governments 2019 fishery product oil price subsidy municipal-level overall funding project, research on artificial breeding and breeding technology of Coelomactra antiquata, Zhan Cainong (2019) No. 152.

语种：英文

外文关键词：Coelomactra antiquata; full-length transcriptome; SMRT sequencing; RNA-seq; function annotation

外文摘要：Coelomactra antiquata is an important aquatic economic shellfish with high medicinal value. However, because C. antiquata has no reference genome, a lot of molecular biology research cannot be carried out, so the analysis of its transcripts is an important step to study the regulatory genes of various substances in C. antiquata. In the present study, we conducted the first full-length transcriptome analysis of C. antiquata by using PacBio single-molecule real-time (SMRT) sequencing technology. The results identified a total of 39,209 unigenes with an average length of 2,732 bp, 23,338 CDSs, 251 AS events, 9,881 lncRNAs, 20,106 SSRs, and 2,316 TFs. Subsequently, 59.22% (23,220) of the unigenes were successfully annotated, of which 23,164, 18,711, 15,840, 13,534, and 13,474 unigenes could be annotated using NR, Swiss-prot, KOG, GO, and KEGG databases, respectively. This study lays the foundation for the follow-up research of molecular biology and provides a reference for studying the more medicinal value of C. antiquata.