文献类型：期刊文献

英文题名：Transcriptome analysis of immune-related gene expression in hybrid snakehead (Channa maculata ♀ x Channa argus ♂) after challenge with Nocardia seriolae

作者：Chen, Jianlin[1,2,3,4];Li, Yanqun[2];Wang, Wenji[1,2,3,4];Xia, Liqun[1,2,4];Wang, Zhiwen[1,2,3,4];Hou, Suying[1,2,3];Huang, Jiahui[2];Lu, Yishan[1,2,3,4]

机构：[1]Guangdong Ocean Univ, Shenzhen Res Inst, 3 Binhai 2nd Rd, Shenzhen 518108, Guangdong, Peoples R China;[2]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang, Peoples R China;[3]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China;[4]Guangdong Prov Engn Res Ctr Aquat Anim Hlth Asses, Shenzhen, Peoples R China

年份：2018

卷号：81

起止页码：476

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000443665900057)、、WOS

基金：We are grateful to all the laboratory members for their constructive suggestions to improve the manuscript. This work was supported by Guangdong Natural Science Foundation (2017A030313179); Shenzhen Science and Technology Project (JCYJ20170306161613251); Shenzhen Dapeng New District Special Fund for Industry Development (KY20160207); International S&T Cooperation Projects of Guangdong Province (2016A050502061); Natural Science Foundation of Guangdong Ocean University (C17377, C13454).

语种：英文

外文关键词：Nocardia seriolae; Hybrid snakehead; Transcriptome analysis; Immune-related genes; Fish nocardiosis

外文摘要：Hybrid snakehead fish (Channa maculata female x Channa cups male), a new species used in freshwater aquaculture in China, is the common host of an epizootic bacterial infection by Nocardia seriolae. However, the information on the functions and mechanisms of hybrid snakehead immune pathways with the N. seriolae infection is limited. Thus, the peripheral blood lymphocytes from hybrid snakehead were used for transcriptome analysis to understand the host immune response after challenge with N. seriolae. A total of 49,839,332 and 50,059,283 raw reads were obtained from the N. seriolae-challenged group (Ns group) and phosphate-buffered saline control group (Ctr group), respectively. The 75.50% and 74.25% reads from the Ns and Ctr groups were matched to reference genomic sequence after cleaning the raw reads, respectively. Additionally, there were 2892 significant differentially expressed genes (DEGs) among the 17,196 expressed genes between the Ns and Ctr groups, including 1387 upregulated and 1505 downregulated genes. All the DEGs were classified into three gene ontology categories, and 2502 DEGs had significant matches, which were allocated to 246 Kyoto Encyclopedia of Genes and Genomes pathways. Immune-related genes were detected from immune system pathways among the top 20 enriched pathways. Moreover, the regulation of several observed effective genes was confirmed by real-time quantitative polymerase chain reaction. Altogether, this study offers deep-sequence data of hybrid snakehead peripheral blood lymphocyte via transcriptome analysis and lays the foundation for further study on the immunogenetics of hybrid snakehead. Moreover, it provides insights into the pathogenic mechanism of N. seriolae, facilitating the prevention and treatment of fish nocardiosis.