文献类型：期刊文献

中文题名：一株新分离的嗜热蓝藻及其藻蓝蛋白的稳定性研究

英文题名：A Newly Isolated of Thermophilic Cyanobacterium and the Stability of Its Phycocyanin

作者：岳瑶[1,2];郭桂筱[1,2];李雁群[1,2,3];胡雪琼[1,2]

机构：[1]广东海洋大学食品科技学院,广东湛江524088;[2]广东海洋大学海洋药物研究所,广东湛江524088;[3]广东省水产品加工与安全重点实验室,广东湛江524088

年份：2022

卷号：43

期号：21

起止页码：159

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD、、EI(收录号：20241015702880)、北大核心、北大核心2020

基金：广东省国际合作项目(2017A050501038)。

语种：中文

中文关键词：嗜热蓝藻;16S rDNA;藻蓝蛋白;稳定性

外文关键词：thermophilic cyanobacterium;16S rDNA;phycocyanin;stability

中文摘要：目的:明确新分离的一株嗜热微藻的分类地位,观察其所含藻蓝蛋白的稳定性,从而了解其藻蓝蛋白可能的应用价值。方法:采用光学显微镜进行细胞形态观察,基于16S rDNA序列建立系统发育树,从而对藻株进行分类鉴定;通过超声波破碎的方法获得藻蓝蛋白粗提液,改变温度、光照强度、pH等条件存放藻蓝蛋白溶液,用分光光度计观察溶液吸光度的变化来反映藻蓝蛋白的稳定性。结果:该藻为杆状单细胞结构,直或有时弯曲,二分裂殖,系统发育树显示该分离藻株为嗜热聚球藻属的一支。提取得到纯度为0.941,色价为196.53的藻蓝蛋白粗提物,得率为11.37%(对藻干物质)。该藻蓝蛋白在60℃保持3.5 h,色值不变;6000 lux光照7 d色值可保持在91.4%左右;在pH4.0~8.0保持3.5 h色值稳定,但在强酸和强碱性环境下藻蓝蛋白的稳定性明显下降。结论:所分离的藻株为嗜热聚球藻属的一支,其所含的藻蓝蛋白具有良好的热、光和酸碱稳定性,在食品加工领域具有良好的应用前景。

外文摘要：Objects:The object of this paper was to determine the taxonomic status of a newly isolated of thermophilic microalga,and to study the stability of its phycocyanin to evaluate its possibility of application in food.Methods:The newly isolated algal strain was morphologically observed by optical microscope,and identified its taxonomic status based on its 16S rDNA sequence and the phylogenetic tree analysis.The phycocyanin of the alga was obtained by ultrasound-assisted extraction,and the stability of phycocyanin was investigated and evaluated based on the changes of the optical density measured with spectrophotometer under different levels of temperature,illumination and pH.Results:The alga cell was rod shape,straight or arc sometimes,binary fission,and was a branch of genus Thermosynechococcus.A crude extract of phycocyanin with a purity of 0.941 and a color value of 196.53 was obtained from the alga,and the yield was 11.37%(for dry mass weight).The stability tests showed that the phycocyanin was stable for 3.5 h at the temperature 60℃,its optical density retained 91.4%after illumination of 6000 lux for 7 d,the color was stable kept pH4.0~8.0 for 3.5 h,but it exhibited a poor stability in strong acid or strong alkali.Conclusion:The isolated microalga is a strain of the genus Thermosynechococcus.Its phycocyanin has good thermal-stability,photo-stability and acid-base-stability,exhibiting encouraging possibility for food application.