文献类型：期刊文献

英文题名：Genome Sequencing and Analysis of Thraustochytriidae sp. SZU445 Provides Novel Insights into the Polyunsaturated Fatty Acid Biosynthesis Pathway

作者：Zhu, Xingyu[1];Li, Shuangfei[1,2,3];Liu, Liangxu[1];Li, Siting[1];Luo, Yanqing[1];Lv, Chuhan[1];Wang, Boyu[1,2,3];Cheng, Christopher H. K.[4];Chen, Huapu[5];Yang, Xuewei[1,2,3]

机构：[1]Shenzhen Univ, Coll Life Sci & Oceanog, Guangdong Key Lab Plant Epigenet, Guangdong Technol Res Ctr Marine Algal Bioengn, Shenzhen 518060, Peoples R China;[2]Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen Key Lab Microbial Genet Engn, Shenzhen Key Lab Marine Biol Resources & Ecol Env, Shenzhen 518055, Peoples R China;[3]Shenzhen Univ, Longhua Innovat Inst Biotechnol, Shenzhen 518060, Peoples R China;[4]Chinese Univ Hong Kong, Sch Biomed Sci, Hong Kong 999077, Peoples R China;[5]Guangdong Ocean Univ, Fisheries Coll, Guangdong Res Ctr Reprod Control & Breeding Techn, Zhanjiang 524088, Peoples R China

年份：2020

卷号：18

期号：2

外文期刊名：MARINE DRUGS

收录：SCI-EXPANDED(收录号：WOS:000518664600049)、、Scopus(收录号：2-s2.0-85079752248)、WOS

基金：This research was funded by [National Key Research and Development Project] grant number [2018YFA0902500], [the Natural Science Foundation of Guangdong Province] grant number [2018A030313139], [Joint R&D Project of Shenzhen-Hong Kong Innovation] grant number [SGLH20180622152010394], [Natural Science Foundation of Shenzhen] grant number [KQJSCX20180328093806045], [Shenzhen Grant Plan for Science & Technology] grant number [CKCY2016042710211071] and [Shenzhen University Start-up Research Funding] grant number [827-000192, 2016100].

语种：英文

外文关键词：whole-genome sequencing; docosahexaenoic acid (DHA); polyunsaturated fatty acid; fatty acid synthesis pathway; polyketide synthase pathway

外文摘要：Thraustochytriidae sp. have broadly gained attention as a prospective resource for the production of omega-3 fatty acids production in significant quantities. In this study, the whole genome of Thraustochytriidae sp. SZU445, which produces high levels of docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA), was sequenced and subjected to protein annotation. The obtained clean reads (63.55 Mb in total) were assembled into 54 contigs and 25 scaffolds, with maximum and minimum lengths of 400 and 0.0054 Mb, respectively. A total of 3513 genes (24.84%) were identified, which could be classified into six pathways and 44 pathway groups, of which 68 genes (1.93%) were involved in lipid metabolism. In the Gene Ontology database, 22,436 genes were annotated as cellular component (8579 genes, 38.24%), molecular function (5236 genes, 23.34%), and biological process (8621 genes, 38.42%). Four enzymes corresponding to the classic fatty acid synthase (FAS) pathway and three enzymes corresponding to the classic polyketide synthase (PKS) pathway were identified in Thraustochytriidae sp. SZU445. Although PKS pathway-associated dehydratase and isomerase enzymes were not detected in Thraustochytriidae sp. SZU445, a putative DHA- and DPA-specific fatty acid pathway was identified.