文献类型：期刊文献

英文题名：Antibacterial Mechanism of Chitosan-Gentamicin and Its Effect on the Intestinal Flora of Litopenaeus vannamei Infected with Vibrio parahaemolyticus

作者：Li, Lefan[1];Liang, Fengyan[1,2,3];Li, Chengpeng[1];Hou, Tingting[1];Xu, Dong-an[1]

机构：[1]Guangdong Ocean Univ, Sch Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[2]LingNan Normal Univ, Life Sci & Technol Sch, Zhanjiang 524048, Peoples R China;[3]LingNan Normal Univ, Mangrove Inst, Zhanjiang 524048, Peoples R China

年份：2022

卷号：20

期号：11

外文期刊名：MARINE DRUGS

收录：SCI-EXPANDED(收录号：WOS:000891893500001)、、Scopus(收录号：2-s2.0-85141892220)、WOS

基金：This work was supported by Guangdong Provincial Natural Science Foundation of China (2020A1515011011) and Mangrove Institute of LingNan Normal University (PYXM10).

语种：英文

外文关键词：chitosan; Vibrio parahaemolyticus; antimicrobial mechanism; intestine microbe

外文摘要：To explore the application of chitosan-gentamicin conjugate (CS-GT) in inhibiting Vibrio parahaemolyticus (V. parahaemolyticus), which is an important pathogen in aquatic animals worldwide, the antimicrobial activity of CS-GT and the effects of a CS-GT dose on the intestine histopathology and intestinal flora of V. parahaemolyticus-infected shrimps were explored. The results showed that CS-GT possessed broad-spectrum antibacterial activity, with minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and half inhibitory concentration (IC50) of 20.00 +/- 0.01, 75.00 +/- 0.02 and 18.72 +/- 3.17 mu g/mL for V. parahaemolyticus, respectively. Further scanning electron microscope and cell membrane damage analyses displayed that the electrostatic interaction of CS-GT with cell membrane strengthened after CS grafted GT, resulting in leakage of nucleic acid and electrolytes of V. parahaemolyticus. On the other hand, histopathology investigation indicated that high (100 mg/kg) and medium (50 mg/kg) doses of CS-GT could alleviate the injury of a shrimp's intestine caused by V. parahaemolyticus. Further 16S rRNA gene sequencing analysis found high and medium dose of CS-GT could effectively inhabit V. parahaemolyticus invasion and reduce intestinal dysfunction. In conclusion, CS-GT possesses good antibacterial activity and could protect shrimps from pathogenic bacteria infection.