文献类型：期刊文献

英文题名：Molecular characterization of CHST11 and its potential role in nacre formation in pearl oyster Pinctada fucata martensii

作者：Wang, Qingheng[1]; Yang, Chuangye[1]; Hao, Ruijuan[1]; Zheng, Zhe[1]; Jiao, Yu[1]; Du, Xiaodong[1]; Deng, Yuewen[1]; Huang, Ronglian[1]

机构：[1] Fishery College, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China

年份：2017

卷号：28

起止页码：113

外文期刊名：Electronic Journal of Biotechnology

收录：EI(收录号：20172803923124)、Scopus(收录号：2-s2.0-85021811852)

语种：英文

外文关键词：Biomineralization - Polymerase chain reaction - Gene expression - RNA - Tissue - Sulfur compounds - Activation analysis

外文摘要：Background C4ST-1 catalyzes the transfer of sulfate groups in the sulfonation of chondroitin during chondroitin sulfate synthesis. Chondroitin sulfate consists of numerous copies of negatively charged sulfonic acid groups that participate in the nucleation process of biomineralization. In the present study, we obtained two CHST11 genes (PmCHST11a and PmCHST11b) which encoded the C4ST-1 and explored the functions of these genes in the synthesis of chondroitin sulfate and in the formation of the nacreous layer of shells. Results Both PmCHST11a and PmCHST11b had a sulfotransferase-2 domain, a signal peptide and a transmembrane domain. These properties indicated that these genes localize in the Golgi apparatus. Real-time PCR revealed that both PmCHST11a and PmCHST11b were highly expressed in the central zone of the mantle tissue. Inhibiting PmCHST11a and PmCHST11b via RNA interference significantly decreased the expression levels of these genes in the central zone of the mantle tissue and the concentration of chondroitin sulfate in extrapallial fluid. Moreover, shell nacre crystallized irregularly with a rough surface after RNA interference. Conclusions This study indicated that PmCHST11a and PmCHST11b are involved in the nacre formation of Pinctada fucata martensii through participating in the synthesis of chondroitin sulfate. ? 2017