文献类型：期刊文献

中文题名：一株纳豆芽孢杆菌的饲料学特性研究

英文题名：The feed Characteristics of a Strain of Bacillus natto

作者：巨向红[1];谢晓杭[1];张金燕[2];徐中文[2];雍艳红[1]

机构：[1]广东海洋大学农学院,广东湛江524088;[2]广东绿百多生物科技有限公司,广东湛江524088

年份：2015

卷号：35

期号：4

起止页码：87

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：CSTPCD

基金：湛江市科技计划项目(2012C0201)

语种：中文

中文关键词：纳豆芽孢杆菌;微生态制剂;饲料学特性;酶活性

外文关键词：Bacillus natto;;probiotics;;feed characteristics;;enzyme activity

中文摘要：测定一株纳豆芽孢杆菌（LBD-3）的耐高温、耐酸、耐胆盐和耐人工胃液能力,以及该菌株发酵饲料的pH、淀粉酶、蛋白酶和纤维酶酶活性.结果表明：该菌株对100℃下10 min 处理、pH 为2 水溶液和人工胃液有较强的耐受能力,但在1 ~ 5 g/L 胆盐环境中存活率低;经其发酵的饲料pH 显著降低（P〈0.05）,淀粉酶活性在第7 和第15 天分别增加6%和20%,而蛋白酶活性则分别增加68%和370%,纤维素酶活性分别提高38%和200%.纳豆芽孢杆菌是较为理想的饲料添加剂用有益微生物,耐受胆盐应作为育种方向.

外文摘要：Choosing a strain of Bacillus natto that has a strong resistance and good effect of feed fermentation and is used in animal feed additives is more and more important. The present study is conducted to detect the capacity of resistance to high temperature, acid bile and artificial gastric juice of a strain of Bacillus natto which are purchased from a biotechnological company. Meanwhile, the p H values, activity of amylase, protease and cellulase enzyme of feed fermented by this species are also observed at 7th to 15 th days after fermentation began. The results showed that this strain of Bacillus natto has a strong tolerance to the environment of 100℃, p H2 and artificial gastric juice. However, the resistant ability is poor to 0.1% to 0.5% bile salt environment. The p H value of feed that are fermented by the bacterial is decreased, but the amylase activity increased to 6% at 7th days and 20% at 15 th day, the protease activity increased to 68% at 7th day and 3.7 fold at 15 th day compared to control(P<0.05), the cellulase activity increased to 38% at 7th days, and around double fold at 15 th days compared to control. Conclusion: This strain of Bacillus natto is an outstanding probiotics for feed additives.