文献类型：期刊文献

英文题名：Transcriptomic analysis of the hepatopancreas response to low-pH stress in kuruma shrimp (Marsupenaeus japonicus)

作者：Hou, Yongkang[1];Liang, Caifeng[1];Kizi, Dekhkonova Dilorakhon Rasuljon[2];Liu, Jianyong[1,3]

机构：[1]Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Guangdong, Peoples R China;[2]Tashkent State Agrarian Univ, Tashkent, Uzbekistan;[3]Guangdong Ocean Univ, Fisheries Coll, 1 Haida Rd, Zhanjiang 524088, Guangdong, Peoples R China

年份：2026

卷号：57

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS

收录：SCI-EXPANDED(收录号：WOS:001615699400001)、、WOS

基金：Chinese Ministry of Science and Technology (2023YFD2401705), Department of Agriculture and Rural Affairs of Guangdong Province, China (2023SDZG01), Development and Utilization of Saline-alkali Land in China-Uzbekistan (080503022501).

语种：英文

外文关键词：Low-pH stress; Transcriptome; Hepatopancreas; Marsupenaeus japonicus; Oxidative stress

外文摘要：Ocean acidification has emerged as a globally recognized environmental issue, posing a serious threat to marine ecosystems. To elucidate the adaptive mechanisms of Marsupenaeus japonicus under acidified conditions, both biochemical and transcriptomic analyses were performed following low-pH exposure. Biochemical assays revealed that low pH stress significantly SOD and CAT activities while markedly elevating MDA levels, indicating oxidative damage. T-AOC exhibited a transient rise followed by a sharp decline at later stages, suggesting initial activation and subsequent exhaustion of antioxidant defense. Transcriptomic profiling identified 2705 DEGs that were primarily enriched in pathways related to immune regulation, redox balance, apoptosis, and energy metabolism, including the lysosome, Toll-like receptor, and PPAR signaling pathways. Protein interaction analysis identified 9 hub genes, including NADH-GOGAT and MDHM, which may play key roles in antioxidant defense and metabolic regulation. The integration of enzyme activity and transcriptomic data indicates that acid stress initially induces oxidative imbalance, followed by compensatory activation of antioxidant and immune systems to restore cellular homeostasis. These findings provide comprehensive insights into the oxidative stress adaptation of M. japonicus and offer a genetic and physiological foundation for breeding acid-tolerant shrimp strains.