文献类型：期刊文献

中文题名：诺氟沙星对土壤微生物碳代谢功能多样性的影响

英文题名：Effect of Norfloxacin on Carbon-source Metabolic Functional Diversity of Soil Microbial Communities

作者：马驿[1];彭金菊[1];陈进军[1];王芸[2];陈法霖[2];孙永学[3]

机构：[1]广东海洋大学农学院动物医学系;[2]中国科学院生态环境研究中心城市与区域生态国家重点实验室;[3]华南农业大学广东省兽药研制与安全评价重点实验室

年份：2012

卷号：18

期号：6

起止页码：1033

中文期刊名：应用与环境生物学报

外文期刊名：Chinese Journal of Applied and Environmental Biology

收录：CSTPCD、、北大核心2011、Scopus、CSCD2011_2012、北大核心、CSCD

基金：国家自然科学基金项目(No.31172368);广东省自然科学基金项目(No.S2012010010628);广东省教育厅广东高校优秀青年创新人才培育项目(No.LYM09087)资助~~

语种：中文

中文关键词：诺氟沙星;土壤微生物群落;碳代谢;功能多样性;生态毒性;BIOLOG检测法

外文关键词：norfloxacin; soil microbial community; carbon-source metabolic capacity; functional diversity; ecological toxicity; BIOLOG

中文摘要：为了解诺氟沙星在环境中的残留对土壤微生物的影响,借助BIOLOG法对诺氟沙星作用下土壤微生物的碳源利用及代谢功能多样性进行了研究.研究发现,用药后d 7、d 21、d 35,用药组(诺氟沙星含量1μg g-1、10μg g-1、100μgg-1)BIOLOG微平板上平均每孔颜色变化率(Average well color development,AWCD)均低于对照组;用药d 7,1μg g-1、10μg g-1、100μg g-1组土壤微生物丰富度指数与多样性指数显著低于(P<0.05)对照组,d 21,10μg g-1、100μg g-1组与对照组差异显著,d 35仅100μg g-1组与对照组差异显著.结果表明,诺氟沙星影响了土壤微生物群落的碳代谢功能,包括代谢强度和代谢多样性,但不同浓度的诺氟沙星对土壤微生物群落代谢功能的影响也不相同.100μg g-1的诺氟沙星在用药的前期和后期均显著影响土壤微生物群落的碳代谢功能,土壤中诺氟沙星积累到该浓度可能对土壤微生物群落碳代谢功能产生难以逆转的长期影响.

外文摘要：The experiment was aimed to investigate the effects of norfloxacin （NOR） on microbial community diversity in soil. The carbon-source metabolic ability and functional diversity of soil microbial communities were analyzed by BIOLOG method. BIOLOG analysis revealed that the average well color development （AWCD） of groups treated with NOR were significantly （P〈0.05） lower than those in control （CK） after 7, 21 and 35 days. The results showed that the richness indexes and diversity indexes of groups treated with NOR concentrations of 1 μg·g^-1, 10 μg·g^-1 and 100 μg·g^-1 were significantly （P〈0.05） lower than those in CK after 7 days, and groups treated with NOR concentrations of 10 μg·g^-1 and 100 μg·g^-1 were significantly lower than those in CK after 21 days, group treated with NOR concentrations of 100 μg·g^-1 was significantly lower than those in CK after 35 days, respectively. It demonstrated that the carbon-source metabolic capacity and functional diversity of soil microbial communities were weaken after soils amended with NOR. However, the effects were different in groups amended with NOR. It was concluded that a certain accumulation of NOR in soil, for instance, 100 μg·g^-1 NOR performed irreversible and long-term effects on the metabolic functions of the soil microbial communities.