文献类型：期刊文献

英文题名：Glucose biosensors based on platinum nanoparticles-deposited carbon nanotubes in sol-gel chitosan/silica hybrid

作者：Kang, Xinhuang[1,2];Mai, Zhibin[1];Zou, Xiaoyong[1];Cai, Peixiang[1];Mo, Jinyuan[1]

机构：[1]Sun Yat Sen Univ, Sch Chem & Chem Engn, Guangzhou 510275, Peoples R China;[2]Guangdong Ocean Univ, Coll Sci, Zhanjiang 524088, Peoples R China

年份：2008

卷号：74

期号：4

起止页码：879

外文期刊名：TALANTA

收录：SCI-EXPANDED(收录号：WOS:000253062400063)、、EI(收录号：20080111006156)、Scopus(收录号：2-s2.0-37449031592)、WOS

语种：英文

外文关键词：glucose; biosensors; carbon nanotubes; platinum nanoparticles; sol-gel; chitosan

外文摘要：A new strategy for fabricating a sensitivity-enhanced glucose biosensor was presented, based on multi-walled carbon nanotubes (CNT), Pt nanoparticles (PtNP) and sol-gel of chitosan (CS)/silica organic-inorganic hybrid composite. PtNP-CS solution was synthesized through the reduction Of PtCl62- by NaBH4 at room temperature. Benefited from the amino groups of CS, a stable PtNP gel was obtained, and a CNT-PtNP-CS solution was prepared by dispersing CNT functionalized with carboxylic groups in PtNP-CS solution. The CS/silica hybrid sol-gel was produced by mixing methyltrimethoxysilane (MTOS) with the CNT-PtNP-CS solution. Then, with the immobilization of glucose oxidase (GOD) into the sol-gel, the glucose biosensor of GOD-CNT-PtNP-CS-MTOS-GCE was fabricated. The properties of resulting glucose biosensor were measured by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). In phosphate buffer solutions (PBS, pH 6.8), nearly interference free determination of glucose was realized at low applied potential of 0.1 V, with a wide linear range of 1.2 x 10(-6) to 6.0 x 10(-3) M, low detection limit of 3.0 x 10(-7) M, high sensitivity of 2.08 mu A mM(-1), and a fast response time (within 5 s). The results showed that the biosensor provided the high synergistic electrocatalytic action, and exhibited good reproducibility, long-term stability. Subsequently, the novel biosensor was applied for the determination of glucose in human serum sample, and good recovery was obtained (in the range of 95-104%). (C) 2007 Elsevier B.V. All rights reserved.