文献类型：期刊文献

中文题名：低电渗流毛细管区带电泳分离芳香胺

英文题名：Separation of Aromatic Amines by Capillary Zone Electro-phoresis with Lower Electroosmotic Flow

作者：黄甫[1];叶盛权[1]

机构：[1]湛江海洋大学应用化学系,广东湛江524006

年份：2004

卷号：22

期号：1

起止页码：77

中文期刊名：色谱

外文期刊名：Chinese Journal of Chromatography

收录：MEDLINE(收录号：15712955)、CSTPCD、、北大核心2000、Scopus(收录号：2-s2.0-54849417802)、CSCD2011_2012、北大核心、CSCD、PubMed

语种：中文

中文关键词：芳香胺;分离;低电渗流毛细管区带电泳;毛细管电泳

外文关键词：lower electroosmotic flow; capillary zone electrophoresis; aromatic amine

中文摘要：利用低pH值(pH≤2 0)有效地抑制电渗流,建立起低电渗流毛细管区带电泳(CZE)体系,并分离了7种芳香胺。在此体系中,芳香胺质子化而带正电荷,故采用在毛细管阳极端进样,阴极端检测。实验考察了pH值、电解质浓度对分离的影响,结果发现,当pH

外文摘要：It is effective to restrain the electroosmotic flow(EOF) with lower pH value (pH≤2.0). As a result, a capillary zone electrophoretic (CZE) system of lower electroosmotic flow was established. Seven aromatic amines were separated by CZE with lower electroosmotic flow. In the system, the aromatic amines combined with protons under the experimental conditions, and had positive charges. Therefore, the sample entered the capillary at the positive electrode and was detected at the negative electrode. It was found that the migration time of aromatic amines increased quickly with a slight increase in pH value in CZE at lower electroosmotic flow. The retention order of peaks of aromatic amines is related to their pK_b and the numbers of amino and acidic groups. When the number of amino groups gets larger, the pK_b becomes smaller, and the peaks elute earlier. The acidic groups of aromatic amines make the peaks to elute later. The optimal conditions were 40 mmol/L NaH_2PO_4( pH 1.7).