文献类型：期刊文献

英文题名：Development of tilapia protein-chondroitin sulfate nanoparticles via pH-driven method for betanin delievery: Focus on stability, bioaccessibility and sustained release

作者：Luo, An[1]; Zhao, Qiaoli[1]; Zhang, Xuan[1]; Wang, Zhuo[1]; Li, Rui[1]; Liu, Qin[3]; Zhong, Saiyi[1,2]

机构：[1] College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, Zhanjiang, 524088, China; [2] Shenzhen Research Institute, Guangdong Ocean University, Shenzhen, 518108, China; [3] Centre for Chinese Medicine Drug Development Limited, Hong Kong Baptist University, Hong Kong

年份：2026

外文期刊名：SSRN

收录：EI(收录号：20260407681)

语种：英文

外文关键词：Biochemistry - Body fluids - Diffusion in liquids - Diffusion in solids - Encapsulation - Nanoparticles - Proteins - Sulfur compounds

外文摘要：Betanin, a natural pigment with potent antioxidant properties, suffers from poor stability that limits its food applications. To address this limitation, a green pH-driven method was developed to fabricate tilapia protein isolated (TPI)-chondroitin sulfate (CS) nanoparticles for betanin encapsulation. The influence of CS concentration (2-10 mg/mL) on nanoparticle formation, physicochemical characteristics, and delivery performance was systematically investigated. Optimal nanoparticles were formed at 2 mg/mL CS, exhibiting the smallest particle size (567.26 nm) and the maximum encapsulation efficiency (81.43%). The nanoparticles displayed enhanced thermal and light stability. During simulated gastrointestinal digestion, the nanoparticles protected betanin in gastric fluid and increased its bioavailability by 2.44-fold. The release kinetics of betanin fit Logistic model, with Fickian diffusion as key mechanism. These findings demonstrate that TPI-CS/Bt nanoparticles fabricated via pH-driven method offer an effective and green strategy for stabilizing and delivering betanin, providing new insights into valorization of aquatic proteins for functional food applications. ? 2026, The Authors. All rights reserved.