文献类型：期刊文献

英文题名：LncRNA LIFR-AS1 promotes proliferation and invasion of gastric cancer cell via miR-29a-3p/COL1A2 axis

作者：Pan, Haiyan[1];Ding, Yuanlin[1];Jiang, Yugang[2];Wang, Xingjie[1];Rao, Jiawei[1];Zhang, Xingshan[1];Yu, Haibing[1];Hou, Qinghua[3];Li, Tao[4]

机构：[1]Guangdong Med Univ, Sch Publ Hlth, Dongguan 523808, Guangdong, Peoples R China;[2]Shandong Prov Hosp, Dept Gastrointestinal Surg, Jinan 250021, Shandong, Peoples R China;[3]Guangdong Ocean Univ, Coll Ocean & Meteorol, Zhanjiang 524088, Guangdong, Peoples R China;[4]Peoples Hosp Gaozhou, Dept Chemotherapy, Gaozhou 525200, Guangdong, Peoples R China

年份：2021

卷号：21

期号：1

外文期刊名：CANCER CELL INTERNATIONAL

收录：SCI-EXPANDED(收录号：WOS:000610115900002)、、Scopus(收录号：2-s2.0-85098775191)、WOS

基金：This work was supported by Medical Science and Technology of Guangdong Province (B2019087), Young Innovative Talents Project of Guangdong Province (2018KQNCX096), Natural Science Foundation of Guangdong Province (2019A1515010875).

语种：英文

外文关键词：LIFR-AS1; miR-29a-3p; COL1A2; Gastric tumor

外文摘要：BackgroundLncRNA was known to be closely associated with the progression of human tumors. The role of lncRNA LIFR-AS1 in the pathogenesis and progression of gastric tumor is still unclear. The aim of this study was to investigate the function of LIFR-AS1 and the underlying mechanism in the pathogenesis and progression of gastric cancer.MethodsQRT-PCR was used to evaluate the expression of LIFR-AS1, miR-29a-3p and COL1A2 in gastric tumor tissues and cells. Western blotting was used to evaluate the protein expression of COL1A2 in gastric tumor cells. CCK-8 assay, transwell assay and flow cytometry were used to evaluate the roles of LIFR-AS1, miR-29a-3p and COL1A2 in cell proliferation, invasion, migration and apoptosis. The relationship among LIFR-AS1, miR-29a-3p and COL1A2 was assessed by bioinformatics analyses and luciferase reporter assay.ResultsThe expression levels of LIFR-AS1 were significantly increased in gastric tumor tissues and cells, while the expression levels of miR-29a-3p were decreased. The expression of miR-29a-3p was negatively correlated with the expression of LIFR-AS1 in gastric cancer tumor tissues. Knocking down of LIFR-AS1 inhibited proliferation, invasion and migration of gastric tumor cells, and induced apoptosis of gastric tumor cells. Bioinformatics analyses and integrated experiments revealed that LIFR-AS1 elevated the expression of COL1A2 through sponging miR-29a-3p, which further resulted in the progression of gastric tumor.ConclusionLIFR-AS1 plays an important role as a competing endogenous RNA in gastric tumor pathogenesis and may be a potential target for the diagnosis and treatment of gastric tumor.