文献类型：期刊文献

英文题名：Comprehensive identification and profiling of Nile tilapia (Oreochromis niloticus) microRNAs response to Streptococcus agalactiae infection through high-throughput sequencing

作者：Wang, Bei[1];Gan, Zhen[1,2];Cai, Shuanghu[1];Wang, Zhongliang[1];Yu, Dapeng[1];Lin, Ziwei[1];Lu, Yishan[1];Wu, Zaohe[1];Jian, Jichang[1]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Key Lab Control Dis Aquat Anim, Guangdong Higher Educ Insititutes,Coll Fishery, Zhanjiang 524025, Peoples R China;[2]Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

年份：2016

卷号：54

起止页码：93

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000378451700012)、、WOS

基金：The authors wish to thank all the laboratory member suggestion on this manuscript. This work supported by National Natural Science Foundation of China (Grant No. 31302226), Science & Technology Planning Project of Guangdong Province of China (Grant No. 2013B020307014) and Project of Enhancing School with Innovation (Grant No. HDYQ2015001). We are especially grateful for the critical comments from the anonymous reviewers. The authors also thank Genedenovo Biothchnology Co., Ltd. (Guangzhou, China) for miRNAs sequence and assisting in the revision of this manuscript.

语种：英文

外文关键词：Oreochromis niloticus; microRNA; Streptococcus agalactiae; Deep sequencing; Immune response

外文摘要：MicroRNAs are a kind of small non-coding RNAs that participate in various biological processes. Deregulated microRNA expression is associated with several types of diseases. Tilapia (Oreochromis niloticus) is an important commercial fish species in China. To identify miRNAs and investigate immune related miRNAs of O. niloticus, we applied high-throughput sequencing technology to identify and analyze miRNAs from tilapia infected with Streptococcus agalactiae at a timescale of 72 h divided into six different time points. The results showed that a total of 3009 tilapia miRNAs were identified, including in 1121 miRNAs which have homologues in the currently available databases and 1878 novel miRNAs. The expression levels of 218 tilapia miRNAs were significantly altered at 6 h-72 h post-bacterial infection (pi), and these miRNAs were therefore classified as differentially expressed tilapia miRNAs. For the 1121 differentially expressed tilapia miRNAs target 41961 genes. GO and KEGG enrichment analysis revealed that some target genes of tilapia miRNAs were grouped mainly into the categories of apoptotic process, signal pathway, and immune response. This is the first report of comprehensive identification of O. niloticus miRNAs being differentially regulated in spleen in normal conditions relating to S. agalactiae infection. This work provides an opportunity for further understanding of the molecular mechanisms of miRNA regulation in O. niloticus host-pathogen interactions. (C) 2016 Elsevier Ltd. All rights reserved.