文献类型：期刊文献

英文题名：Comprehensive whole transcriptome analysis reveals specific lncRNA, circRNA and miRNA-mRNA networks of pearl oyster (Pinctada fucata martensii) in response to hypoxia stress

作者：Wu, Qian[1];Zhang, Jiawei[1,4,6];Liu, Lintao[1];Yang, Chuangye[1,2,3,4];Liao, Yongshan[5];Wang, Qingheng[1,2,3,4,5];Deng, Yuewen[1,2,3,4,5]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Guangdong Sci & Innovat Ctr Pearl Culture, Zhanjiang 524088, Peoples R China;[3]Pearl Breeding & Proc Engn Technol Res Ctr Guangdo, Res Ctr Guangdong Prov, Zhanjiang 524088, Peoples R China;[4]Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China;[5]Guangdong Ocean Univ, Pearl Res Inst, Zhanjiang 524088, Peoples R China;[6]Key Lab Marine Ecol & Aquaculture Environm Zhanjia, Zhanjiang 524033, Peoples R China

年份：2026

卷号：46

外文期刊名：AQUACULTURE REPORTS

收录：SCI-EXPANDED(收录号：WOS:001648871000001)、、WOS

基金：This research was funded by Shellfish & Algae Industry InnovationTeam of Guangdong Modern Agricultural Technology System (Grant no. 2024CXTD23) , Research on industrial innovation technology for Guangdong modern marine ranching (Grant no. 2024-MRI-001-03) , the Undergraduate Innovation Team of Guangdong Ocean University (Grant no. CXTD2025001) , the Department of Education of Guangdong Province (Grant no. 2021KCXTD026) , and the program for scientific research start-up funds of Guangdong Ocean University (Grant no. 060302022304) .

语种：英文

外文关键词：Hypoxia; Pearl oyster; Competitive endogenous RNAs; Metabolism; Energy compensation

外文摘要：Hypoxia is a prevalent environmental stressor in both natural and aquaculture environments. Pinctada fucata martensii, a commercially valuable pearl oyster species, is particularly susceptible to hypoxic stress in aquaculture. To explore its molecular response to short-term hypoxia, whole-transcriptome sequencing was performed on gill tissues under normoxic and hypoxic conditions. A total of 721 differentially expressed mRNAs (DEmR-NAs), 259 long non-coding RNAs (DElncRNAs), 55 circular RNAs (DEcircRNAs), and 17 microRNAs (DEmiRNAs) were identified. Based on predicted interactions among these differentially expressed RNAs, a competing endogenous RNA (ceRNA) network was constructed, consisting of 9 DElncRNAs, 10 DEcircRNAs, 3 DEmiRNAs, and 6 DEmRNAs. Six DElncRNAs and three DEcircRNAs were predicted to interact with miR-152, and the axis-mediated activation of target genes-ALDH5A1 and SLC23A2, FAT4 and CCDC97, and LAC-was implicated in the modulation of metabolic pathways, enzyme activity, antioxidant defenses, thereby promoting antioxidant capacity and suppressing cell growth as part of an adaptive survival strategy. This ceRNA network reveals a key molecular hub that links hypoxia sensing to immunomodulatory mechanisms. Moreover, the co-repression of stress-response genes HSP20 and HSP70 in this network enhances hypoxic tolerance increased energy utilization efficiency and regulation of RNA. Functional analysis of the network revealed enrichment in pathways related to energy metabolism, antioxidant defense, and RNA splicing regulation. These findings provide novel insights into transcriptomic regulation and adaptive mechanisms in bivalves under hypoxic stress.