文献类型：期刊文献

中文题名：基于全基因组重测序技术的狮头鹅Indel标记分析

英文题名：Analysis of Indel Markers of Shitou Goose Based on Whole Genome Resequencing Technology

作者：王慧芳[1];周光现[1];孙永峰[2];陈新企[1];雷栩斌[1];张锐毅[1];贾汝敏[1];赵志辉[1]

机构：[1]广东海洋大学滨海农业学院,湛江524088;[2]吉林农业大学动物科学技术学院,长春130118

年份：2021

卷号：52

期号：3

起止页码：662

中文期刊名：畜牧兽医学报

外文期刊名：ACTA VETERINARIA ET ZOOTECHNICA SINICA

收录：CSTPCD、、CSCD2021_2022、北大核心、CSCD、北大核心2020

基金：广东省现代农业产业技术体系创新团队(2019KJ137)。

语种：中文

中文关键词：狮头鹅;重测序;Indel;信号通路;产蛋

外文关键词：Shitou goose;resequencing;Indel;signaling pathway;egg-laying

中文摘要：旨在利用重测序技术分析狮头鹅基因Indel差异并探讨其产蛋调控通路。本研究选用产蛋量有差异的成年狮头鹅和吉林白鹅母鹅各5只,采用全基因组重测序方法,对两个品种基因组水平的插入缺失突变情况进行比较分析。结果表明,测序共得到350.35 Gb数据,其中Q20与Q30平均值分别为96.74%、92.19%;通过注释分析发现了748821.2个Indel变异位点。10只试验鹅的Indel位点在编码区(CDS)的分布趋势和数量与参考基因组相似,其中有11737个Indel位点导致了插入或删除;在此基础上,进一步分析筛选产蛋相关通路,发现在GnRH信号通路、雌激素信号通路、催产素信号通路、催乳素信号通路以及孕酮介导的卵母细胞成熟通路中分别有15、1、1、1、18个变异基因参与狮头鹅产蛋调控。结果显示,通过Indel位点分析表明,GnRH信号通路、雌激素信号通路、催产素信号通路、催乳素信号通路和孕酮介导的卵母细胞成熟通路均与狮头鹅产蛋有关,并进一步筛选出可能的产蛋相关基因。这些数据将为我们研究狮头鹅繁殖性能提供有效基础和依据。

外文摘要：This study aimed to use resequencing technology to analyze the differences in Shitou goose gene Indel and to explore its egg production regulatory pathway.Five adult Shitou geese and five Jilin white geese with different egg production were selected,and the whole genome resequencing method was used to compare and analyze the indel mutations at the genome level of the two breeds.The results showed that 350.35 Gb data was obtained by sequencing,among which the average values of Q20 and Q30 reached 96.74%and 92.19%,respectively.Annotation analysis found 748821.2 Indel mutation sites.The distribution trend and the number of Indel loci in the coding region(CDS)of 10 experimental geese were similar to those of the reference genome.Among them,11737 Indel loci resulted in insertion or deletion.On the basis,egg-laying related pathways were further analyzed and screened,and 15,1,1,1,and 18 variant genes in the GnRH,estrogen,oxytocin,prolactin signaling pathways and progesterone-mediated oocyte maturation pathway,respectively involved in the regulation of goose egg-laying were found.The results suggest that the GnRH,estrogen,oxytocin,prolactin signaling pathways and progesterone-mediated oocyte maturation pathway are all related to Shitou goose egg-laying,and egg-laying related genes were further screened.These data will provide an effective basis for studying the reproductive performance of Shitou geese.