文献类型：期刊文献

中文题名：低鱼粉饲料中补充蛋氨酸对军曹鱼生长性能、体成分及肌肉氨基酸组成的影响

英文题名：Effects of Methionine Supplementation in Low Fish Meal Diet on Growth Performance,Body Composition and Muscle Amino Acid Composition of Cobia (Rachycentron canadum)

作者：何远法[1];郭勇[1];迟淑艳[1];谭北平[1,2];董晓慧[1,2];杨奇慧[1];刘泓宇[1];章双[1]

机构：[1]广东海洋大学水产学院;[2]南海生物资源开发与利用协同创新中心

年份：2018

卷号：30

期号：2

起止页码：624

中文期刊名：动物营养学报

外文期刊名：Chinese Journal of Animal Nutrition

收录：CSTPCD、、北大核心2017、CSCD2017_2018、北大核心、CSCD

基金：国家自然科学基金项目(31402310);现代农业产业技术体系专项资金资助项目(CARS-49)

语种：中文

中文关键词：军曹鱼;蛋氨酸;生长性能;体成分;肌肉氨基酸含量

外文关键词：cobia; methionine; growth performance; body composition; muscle amino acid composition

中文摘要：本试验旨在研究低鱼粉饲料中补充蛋氨酸对军曹鱼(Rachycentron canadum)生长性能、体成分及肌肉氨基酸组成的影响。在低鱼粉饲料中添加DL-蛋氨酸,配制蛋氨酸水平分别为0.72%、0.90%、1.00%、1.24%、1.41%、1.63%和1.86%的7种等氮等脂饲料。选取初始体重为(9.79±0.04)g的军曹鱼840尾,随机分为7组,每组3个重复,每个重复40尾鱼,进行为期16周的饲养试验。结果表明:0.90%和1.00%组的成活率显著高于0.72%组(P<0.05)。随着饲料中蛋氨酸水平的升高,军曹鱼的增重率、特定生长率、蛋白质效率均呈先升高后降低的变化趋势,在1.00%组达到最大值,显著高于其余各组(P<0.05)。1.00%组的饲料系数最低,除与0.90%和1.24%组差异不显著(P>0.05)外,显著低于其余各组(P<0.05)。0.72%组全鱼粗蛋白质含量显著低于其余各组(P<0.05);0.90%组全鱼粗脂肪含量达到最高,除与0.72%和1.00%组差异不显著(P>0.05)外,显著高于其余各组(P<0.05);1.24%组全鱼粗灰分含量显著高于0.72%和0.90%组(P<0.05)。随着饲料蛋氨酸水平的升高,军曹鱼肌肉中苯丙氨酸、赖氨酸、亮氨酸、丙氨酸、蛋氨酸以及必需氨基酸和总氨基酸含量均无显著差异(P>0.05),但1.00%组肌肉中苏氨酸、缬氨酸、异亮氨酸、组氨酸含量显著高于1.63%组(P<0.05)。由此可见,在低鱼粉饲料中补充蛋氨酸可提高军曹鱼的生长性能和体蛋白质含量;以增重率作为评价指标,经二次回归分析可知,军曹鱼对饲料中蛋氨酸的需要量为1.12%(占饲料蛋白质的2.43%)。

外文摘要：A 16-week feeding trial was carried out to investigate the effects of methionine supplementation in low fish meal diet on growth performance, body composition and muscle amino acid composition of cobia （Rachycentron canadum）. Seven isonitrogenous and isolipidic diets were prepared by adding DL-methionine in a diet with low fish meal, and the methionine level in the seven diets was 0.72%, 0.90%, 1.00%, 1.24%, 1.41%, 1.63% and 1.86%, respectively. A total of 840 cobia with an initial body weight of （9.79_＋0.04） g were randomly assigned into 7 groups with 3 replicates per group and 40 individuals per replicate. The results showed that the survival rate （SR） in 0.90% and 1.00% groups was significantly higher than that in 0.72% group （P〈0.05）. The weight gain rate （WGR）, specific growth rate （SGR） and protein efficiency ratio （PER） were increased firstly and then decreased as dietary methionine level increasing, and the WGR, SGR and PER in 1.00% group had the highest values which were significantly higher than those in other groups （ P〈 0.05）. The feed conversation ratio （FCR） in 1.00% group had the lowest value, which was significantly low- er than that in other groups except 0.90% and 1.24% groups （P〈0.05）. The crude protein content of whole body in 0.72% group was significantly lower than that in other groups （P〈0.05）. The maximum crude lipid content was observed in 0.90% group, and the difference was significant compared with the other groups ex- cept 0.72% and 1.00% groups （P〈0.05）. The ash content of whole body in 1.24% group was significantly higher than that in 0.72% and 0.90% groups （P〈0.05）. The contents of phenylalanine, lysine, leucine, ala- nine, methionine, essential amino acid and total amino acid in muscle had no significant differences with dieta- ry methionine level increasing （P〉0.05）, but the contents of threonine, valine, isoleucine and histidine in muscle in 1.00% group were significantly higher than those in 1.63% group （P〈0.05）. It can be concluded that the supplementation of methionine in low fish meal diet can significantly enhance the growth performance and body protein content of cobia, since the methionine requirement of cobia is 1.12% of diet （2.43% of dieta- ry protein） which is estimated by quadratic regression analysis with WGR as the evaluation index.