文献类型：期刊文献

英文题名：Orange-spotted grouper (Epinephelus coioides) toll-like receptor 22: Molecular characterization, expression pattern and pertinent signaling pathways

作者：Ding, Xu[1,2,3];Lu, Dan-qi[1,2];Hou, Qing-hua[1,2,4];Li, Shui-sheng[1,2];Liu, Xiao-chun[1,2];Zhang, Yong[1,2];Lin, Hao-ran[1,2,3]

机构：[1]Sun Yat Sen Zhongshan Univ, Coll Life Sci, Inst Aquat Econ Anim, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China;[2]Sun Yat Sen Zhongshan Univ, Coll Life Sci, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou 510275, Guangdong, Peoples R China;[3]Hainan Univ, Ocean Coll, Haikou 570228, Hainan, Peoples R China;[4]Guangdong Ocean Univ, Coll Ocean & Meteorol, Zhanjiang 524025, Guangdong, Peoples R China

年份：2012

卷号：33

期号：3

起止页码：494

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000308057700004)、、WOS

基金：This project was supported by the National 863 Program of China (No. 2012AA10A407 and 2012AA092201), the Special Fund for Agro-scientific Research in the Public Interest (No. 200903046), Jiangsu Provincial Science and Technology Achievements Program Special Fund (No.BA2009083), the National Natural Science Foundation of China (Grant No. 31001126), Specialized Research Fund for the Doctoral Program of Higher Education (Grant No. 20110171130005) the Guangdong Provincial Science and Technology Program (No. 20108090400551, 20118020307009), and the Guangdong Natural Science Foundation (Grant No.10451027501005658).

语种：英文

外文关键词：Orange-spotted grouper (Epinephelus coioides); Toll-like receptor 22 (TLR22); Expression analysis; Signaling pathway

外文摘要：The toll-like receptors (TLRs) are an important gene family in host innate immunologic surveillance. The TLR22 gene is an essential member of the TLRs that is only found in aquatic animals and has been detected in some bony fish. Here, a TLR22 homolog, EcTLR22, was characterized in the orange-spotted grouper (Epinephelus coioides) via homology cloning. The 3321 bp full-length cDNA sequence of EcTLR22 was obtained, which included an open reading frame of 2880 bp encoding a putative peptide of 960 amino acids containing three highly typical domains with the characteristics of TLR family members. The deduced amino acid sequence of EcTLR22 showed a relatively high similarity to flounder TLR22. Phylogenetic analysis showed that the orange-spotted grouper TLR22 sequence was clustered with those of Perciforme, such as flounder and croaker. Real-time quantitative PCR analysis revealed broad expression of EcTLR22, with relatively high expression detected in the head kidney, trunk kidney, spleen, peripheral blood leukocytes (PBLs) and heart of orange-spotted grouper. After injection with Vibrio alginolyticus, there was significant up-regulation of the expression of EcTLR22 in the spleen. In evaluating unstimulated/stimulated head kidney leukocytes and spleen leukocytes, a significant increase in EcTLR22 mRNA expression was detected, which implied a sensitive immune response. Furthermore, four important molecules for signal transduction, MyD88, TRIF, TNF-alpha and IRF3, were chosen to analyze the role of the EcTLR22 signaling pathway in anti-pathogen responses. Upon LPS or Poly I:C challenge, expression of the four genes was induced, with an increasing tendency detected in head kidney leukocytes, suggesting that the four genes might work with EcTLR22 in host defense against pathogenic microbes. (C) 2012 Elsevier Ltd. All rights reserved.