文献类型：期刊文献

英文题名：L-Cysteine hydrochloride inhibits Aspergillus flavus growth and AFB1 synthesis by disrupting cell structure and antioxidant system balance

作者：Qiu, Mei[1];Qiu, Lihong[2];Deng, Qi[1];Fang, Zhijia[1];Sun, Lijun[1];Wang, Yaling[1];Gooneratne, Ravi[3];Zhao, Jian[4]

机构：[1]Guangdong Ocean Univ, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang 524088, Peoples R China;[2]Guangdong Med Univ, Affiliated Hosp, Dept Clin Lab, Zhanjiang 524001, Guangdong, Peoples R China;[3]Lincoln Univ, Fac Agr & Life Sci, Dept Wine Food & Mol Biosci, Lincoln 7647, New Zealand;[4]Univ New South Wales, Sch Chem Engn, Sydney, NSW 2052, Australia

年份：2023

卷号：459

外文期刊名：JOURNAL OF HAZARDOUS MATERIALS

收录：SCI-EXPANDED(收录号：WOS:001055969200001)、、WOS

基金：This work was supported by the National Natural Science Foundation of China (No. 31871898) , Agricultural Science and Technology Devel- opment and Resource Environmental Protection Management Project (No. 2023KJ149 and 2023KJ151) , Guangdong Provincial Key Research and Development Program (No. 2021B0202060001) , Innovative Team Program of High Education of Guangdong Province (No. 2021KCXTD021) , Characteristic Innovation Project of Colleges and Universities in Guangdong Province (No. 2020KQNCX025) , and Zhan- jiang Science and Technology Development Special Fund Competitive Allocation Project (No. 2020A03009 and 2020A01011) .

语种：英文

外文关键词：L-Cysteine hydrochloride; Aspergillus flavus; Aflatoxin; Dwarf mycelium

外文摘要：Aflatoxin B1 (AFB1) is the most potent known naturally occurring carcinogen and pose an immense threat to food safety and human health. L-Cysteine hydrochloride (L-CH) is a food additive often used as a fruit and vegetable preservative and also to approved bread consistency. In this study, we investigated the effects and mechanisms of L-CH as an antimicrobial on the growth of Aspergillus flavus (A. flavus) and AFB1 biosynthesis. L-CH significantly inhibited A. flavus mycelial growth, affected mycelial morphology and AFB1 synthesis. Furthermore, L-CH induced glutathione (GSH) synthesis which scavenged intracellular reactive oxygen species (ROS). RNA-Seq indicated that L-CH inhibited hyphal branching, and spore and sclerotia formation by controlling cell wall and spore development-related genes. Activation of the GSH metabolic pathway eliminated intracellular ROS, leading to hyphal dwarfing. L-CH treatment downregulated most of the Aflatoxin (AF) cluster genes and aflS, aflR, AFLA_091090 transcription factors. This study provides new insights into the molecular mechanism of L-CH control of A. flavus and AFB1 foundation. We believe that L-CH could be used as a food additive to control AFB1 in foods and also in the environment.