文献类型：期刊文献

中文题名：多鳞鱚(Sillago sihama)4个野生地理群体的微卫星标记分析

英文题名：SSR ANALYSIS IN FOUR WILD POPULATIONS OF SILVER SILLAGO SILLAGO SIHAMA

作者：郭昱嵩[1];颜程钟[1];张宇岚[1];郑近楠[1];许远民[1];杜涛[1];王中铎[1];刘楚吾[1]

机构：[1]广东海洋大学水产学院南海水产经济动物增养殖广东普通高校重点实验室,湛江524088

年份：2013

卷号：44

期号：2

起止页码：267

中文期刊名：海洋与湖沼

外文期刊名：Oceanologia Et Limnologia Sinica

收录：CSTPCD、、北大核心2011、Scopus、北大核心、CSCD、CSCD2013_2014

基金：国家自然科学基金资助;31101904号;31201996号;广东省科技计划项目资助;2010A020307001-84号;广东省大学生创新实验项目;1056610003号

语种：中文

中文关键词：多鳞[鱼]喜;微卫星DNA;遗传多样性;遗传分化

外文关键词：Sillago sihama; microsatellite DNA; genetic diversity; genetic differentiation

中文摘要：采用微卫星DNA分子标记方法对中国南部沿海多鳞鱚(Sillago sihama)广西北海、广东湛江、汕尾及海南三亚4个野生地理群体进行了遗传多样性及遗传分化等研究。结果表明:(1)用于研究的34对微卫星引物在4个群体中具有丰富的多态性,有效等位基因数介于1.71—20.46之间,多态信息含量PIC介于0.40—0.95之间。4个群体总的平均期望杂合度(He)为0.598,观测杂合度(Ho)为0.572,SY群体的杂合度最大(He=0.606,Ho=0.623),SW群体的最小(He=0.586,Ho=0.508)。(2)群体间F-统计量分析表明,群体遗传分化处于高度分化水平(Fst平均值为0.306);基于奈氏遗传距离(DA)构建的NJ和UPGMA聚类树均显示地理位置相邻的群体聚在一起。

外文摘要：Sillago sihama is the most known species in genus Sillago. It is naturally distributed in Indo-West Pacific and found in all Chinese waters including East China Sea, Yellow Sea, Bohai Sea, South China Sea, and the Northern Bay. In China, it is a common marine food fish species, and the supply of this fish relies on capture from the wild. The resources have reduced dramatically. It is necessary to reveal genetic diversity and population structure of wild stocks before carry out breeding and farming. Genetic diversity of 4 wild geographical populations of S. sihama in the southern coast waters of China, i.e. four populations of Zhanjiang （ZJ）, Beihai （BH）, Shanwei （SW）, and Sanya （SY）, were investigated using 34 microsatellite DNA loci. The allele numbers （Na）, effective allele numbers （Are）, expected heterozygosity （He）, observed heterozygosity （Ho）, polymorphism information contents （PIC） and Hardy-Weinberg equilibrium were estimated to char- acterize genetic diversity. The results show that all loci were polymorphic among populations. Test of departures from Hardy-Weinberg equilibrium indicated that 7 to 12 loci in each population were significantly deviated from Hardy- Weinberg equilibrium. All loci are not significant deviation from Hardy-Weinberg equilibrium in all 4 populations. The Are ranged from 1.71 to 20.46, and PIC ranged from 0.40 to 0.95. The average He was 0.598, and average Ho was 0.572 in four populations. The SY population showed the highest heterozygosity （He = 0.606, Ho = 0.623）, while the SW population showed the lowest value （He = 0.586, Ho = 0.508）. The results of genetic distances and genetic similarity show that the genetic relationship of BH and ZJ populations was the most closed, while that of SY and SW populations was opposite. The F-statistics analysis showed that the four populations were highly differentiated （Fst mean value is 0.306）. There was only a very small gene flow between populations. NJ and UPGMA clustering tree based on DA genetic distances demonstrated that the stocks of adjacent geographical position clustered together.