文献类型：期刊文献

英文题名：Highly regioselective synthesis of betulone from betulin by growing cultures of marine fungus Dothideomycete sp HQ 316564

作者：Liu, Huan[1];Lei, Xiao-Ling[2];Li, Ning[1];Zong, Min-Hua[1]

机构：[1]S China Univ Technol, State Key Lab Pulp & Paper Engn, Coll Light Ind & Food Sci, Guangzhou 510640, Peoples R China;[2]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524025, Guangdong, Peoples R China

年份：2013

卷号：88

起止页码：32

外文期刊名：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

收录：SCI-EXPANDED(收录号：WOS:000315758400006)、、WOS

基金：This work is financially supported by the Science and Technology Project of Guangdong Province (2010B011000003), the National Natural Science Foundation of China (20906032 and 21072065) and the Research Fund for the Doctoral Program of Higher Education (20090172110019).

语种：英文

外文关键词：Biotransformation; Natural products; Pentacyclic triterpenes; Regioselective oxidation; Whole cells

外文摘要：The synthesis of betulone from betulin through highly regioselective oxidation catalyzed by growing microorganism cells was reported for the first time. Fifty-two microbial strains including many marine fungi were examined for their capability of transformation of betulin and betulinic acid. Among the strains tested, the marine fungus Dothideomycete sp. HQ 316564 was the unique strain that regioselectively catalyzed the oxidation of betulin to betulone and the regioselectivity toward 3-hydroxyl was exclusive; nonetheless, no strains could transform betulinic acid into its 3-carbonyl derivative betulonic acid. The effects of several key culture and reaction conditions on the synthesis of betulone were studied. The optimum initial culture pH, reaction temperature and the concentration of the co-solvent dimethyl sulfoxide were 6.0, 33 degrees C and 0.4% (v/v), respectively, under which the yield of betulone reached 43.4%. (c) 2012 Elsevier B.V. All rights reserved.