文献类型：期刊文献

英文题名：Squid ink polysaccharide prevents autophagy and oxidative stress affected by cyclophosphamide in Leydig cells of mice: a pilot study

作者：Gu, Yi-Peng[1,2,3];Yang, Xiao-Mei[1];Duan, Zhen-Hua[1];Shang, Jiang-Hua[4];Luo, Ping[2];Xiao, Wei[3];Zhang, Da-Yan[3];Liu, Hua-Zhong[2]

机构：[1]Hezhou Univ, Coll Food & Biol Engn, Hezhou 542899, Peoples R China;[2]Guangdong Ocean Univ, Coll Chem & Environm, Zhanjiang 524088, Peoples R China;[3]Guangdong Ocean Univ, Sch Food Sci & Technol, Zhanjiang 524088, Peoples R China;[4]Chinese Acad Agr Sci, Guangxi Buffalo Res Inst, Nanning 530001, Peoples R China

年份：2017

卷号：20

期号：11

起止页码：1194

外文期刊名：IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES

收录：SCI-EXPANDED(收录号：WOS:000417394100003)、、Scopus(收录号：2-s2.0-85035146880)、WOS

基金：This work was supported by the National Natural Science Foundation of China (31171667), Special Fund for Distinguished Experts in Guangxi and Guangxi talent highland of preservation and deep processing research in fruit and vegetables. The results presented in this paper were part of a student thesis.

语种：英文

外文关键词：Autophagy; Cyclophosphamide; Leydig cells; Oxidative stress; Squid ink polysaccharide

外文摘要：Objective(s): The aim of this study was to explore the effects of Squid ink polysaccharide (SIP) on prevention of autophagy and oxidative stress induced by cyclophosphamide (CP) in Leydig cells of mice. Materials and Methods: Examination of reproductive organ exponents, abnormal sperm rate, activities of superoxide dismutase (SOD), catalase (CAT), contents of malondialdehyde (MDA), and histological structure were performed to detect the optimal dose of SIP against oxidative stress damage in vivo, and autophagyassociated protein LC3 and Beclin-1 were examined by immunofluorescence, and their expression was detected by Western blot analysis. Leydig cells ultrastructural changes were observed by transmission fluorescent microscope. Results: SIP significantly inhibited sperm aberration, histological structure and injury of seminiferous tubules caused by CP, as well as the antioxidant activity of SOD and CAT were increased; contents of MDA were decreased. The optimal dose of SIP for prevention of oxidative stress injury by CP was 80 mg/kg. In addition, LC3 and Beclin-1 fluorescent granules were much less in the Leydig cell layer after treatment via SIP compared with the CP-treated group, and the expression levels of LC3 and Beclin-1 were also decreased. Furthermore, characteristics of cell autophagy such as mitochondrial swelling, autophagic vacuoles, and chromatin pyknosis were observed in CP-treated Leydig cells, but SIP could effectively weaken injury of Leydig cell ultrastructure by CP. Conclusion: SIP, as an antioxidant, prevents the cytoskeleton damage through up-regulation antioxidant capacity and inhibition autophagy caused by CP.