文献类型：期刊文献

英文题名：Effects of Chronic Heat Stress on Growth, Apoptosis, Antioxidant Enzymes, Transcriptomic Profiles, and Immune-Related Genes of Hong Kong Catfish (Clarias fuscus)

作者：Liu, Yong[1];Tian, Changxu[1];Yang, Zhihua[1];Huang, Cailin[2];Jiao, Kaizhi[1];Yang, Lei[1];Duan, Cunyu[1];Zhang, Zhixin[2];Li, Guangli[1]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Guangdong Res Ctr Reprod Control & Breeding Techno, Guangdong Prov Engn Lab Mariculture Organism Breed, Zhanjiang 524088, Peoples R China;[2]Guangxi Intro & Breeding Ctr Aquaculture, Nanning 530001, Peoples R China

年份：2024

卷号：14

期号：7

外文期刊名：ANIMALS

收录：SCI-EXPANDED(收录号：WOS:001201567900001)、、WOS

基金：We wish to thank the anonymous reviewers and the editor of the journal for their valuable comments.

语种：英文

外文关键词：transcriptome; oxidative stress; chronic heat stress; apoptosis; Clarias fuscus

外文摘要：Chronic heat stress can have detrimental effects on the survival of fish. This study aimed to investigate the impact of prolonged high temperatures on the growth, antioxidant capacity, apoptosis, and transcriptome analysis of Hong Kong catfish (Clarias fuscus). By analyzing the morphological statistics of C. fuscus subjected to chronic high-temperature stress for 30, 60, and 90 days, it was observed that the growth of C. fuscus was inhibited compared to the control group. The experimental group showed a significant decrease in body weight and body length compared to the control group after 60 and 90 days of high-temperature stress (p < 0.05, p < 0.01). A biochemical analysis revealed significant alterations in the activities of three antioxidant enzymes superoxide dismutase activity (SOD); catalase activity (CAT); glutathione peroxidase activity (GPx), the malondialdehyde content (MDA), and the concentrations of serum alkaline phosphatase (ALP); Aspartate aminotransferase (AST); and alanine transaminase (ALT) in the liver. TUNEL staining indicated stronger apoptotic signals in the high-temperature-stress group compared to the control group, suggesting that chronic high-temperature-induced oxidative stress, leading to liver tissue injury and apoptosis. Transcriptome analysis identified a total of 1330 DEGs, with 835 genes being upregulated and 495 genes being downregulated compared to the control group. These genes may be associated with oxidative stress, apoptosis, and immune response. The findings elucidate the growth changes in C. fuscus under chronic high temperature and provide insights into the underlying response mechanisms to a high-temperature environment.