文献类型：期刊文献

中文题名：Effects of hydroxyproline supplementation in low fish meal diets on collagen synthesis,myofiber development and muscular texture of juvenile Pacific white shrimp(Litopenaeus vannamei)

作者：Menglin Shi[1];Haoming Li[1];Tianyu Chen[1];Bocheng Huang[1];Xiaoyue Li[1];Xiaohui Dong[1,2,3];Shuyan Chi[1,2,3];Qihui Yang[1,2,3];Hongyu Liu[1,2,3];Junming Deng[1,2,3];Beiping Tan[1,2,3];Shuang Zhang[1,2,3];Shiwei Xie[1,2,3,4]

机构：[1]Laboratory of Aquatic Nutrition and Feed,College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China;[2]Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province,Zhanjiang 524088,China;[3]Key Laboratory of Aquatic,Livestock and Poultry Feed Science and Technology in South China,Ministry of Agriculture,Zhanjiang 524088,China;[4]Guangdong Provincial Key Lab of Aquatic Animals Disease Control and Healthy Culture,Zhanjiang 524088,China

年份：2024

期号：2

起止页码：428

中文期刊名：Animal Nutrition

外文期刊名：动物营养（英文版）

收录：CSTPCD、、Scopus、CSCD2023_2024、CSCD、PubMed

基金：supported by the fund of the National Natural Science Foundation of China(32373141);the Guangdong Basic and Applied Basic Research Foundation(2023A1515030007);ModernSeed Industry Park for Whiteleg Shrimp of Guangdong Province,program for scientific research start-up funds of Guangdong Ocean University,Science and Technology Bureau of Zhanjiang(2020A05003,2022A 01002).

语种：英文

中文关键词：Hydroxyproline;Collagen;Muscle quality;Litopenaeus vannamei

中文摘要：This experiment aimed to evaluate the impact of dietary hydroxyproline(Hyp)supplementation on the muscle quality of juvenile Pacific white shrimp(Litopenaeus vannamei)fed a low fishmeal diet.Six formulated diets included one high fishmeal(HF;25%fishmeal content)and five low fishmeal diets(10%fishmeal content)with 0%,0.2%,0.4%,0.6%and 0.8%Hyp(LFO,LF2,LF4,LF6 and LF8,respectively).Each diet was assigned to four replicates,and 40 shrimp(0.32±0.00 g)per replicate were fed four times a day for 8 weeks.Dietary Hyp supplementation had little effects on growth performance,but increased the contents of Hyp,prolyl 4-hydroxylases(P4Hs),and collagen.The meat yield,springiness,hardness,chewiness,and cohesiveness of muscle were the highest in the LF4 group among the low fishmeal groups(P<0.05).Cooking loss and freezing loss of muscle were the lowest in the LF4 group(P<0.05).Dietary supplementation with 0.4%Hyp increased the myofiber density and decreased the myofiber diameter of muscle(P<0.05).Supplementation of Hyp in the diet up-regulated the mRNA expression of smyhc5,smyhc15,col1a1,col1a2,igf-1f,tgf-β and tor and down-regulated the mRNA expression of smy hc1,smyhc2,smyhc6a(P<0.05).Supplementation of Hyp in the diet up-regulated the protein expression of P-4E-BP1,P-AKT,AKT and P-AKT/AKT(P<0.05).These results suggested that the addition of 0.4%Hyp to low fishmeal diets improved the muscle quality of L.vannamei.