文献类型：期刊文献

中文题名：牡蛎锌结合肽的稳定性及其跨Caco-2细胞模型的吸收转运机制

英文题名：Stability of Oyster Zinc-binding Peptides and Their Mechanism of Absorption and Transport Across Caco-2 cell Monolayers

作者：杜冉[1];陈忠琴[1];谭明堂[1];高加龙[1];林海生[1];郑惠娜[1];曹文红[1]

机构：[1]广东海洋大学食品科技学院/国家贝类加工技术研发分中心(湛江)/广东省水产品加工与安全重点实验室/广东省海洋生物制品工程实验室/广东省海洋食品工程技术研究中心/水产品深加工广东普通高等学校重点实验室,广东湛江524088

年份：2024

卷号：44

期号：3

起止页码：103

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：北大核心2023、CSTPCD、、CSCD_E2023_2024、北大核心、CSCD

基金：湛江市农业技术攻关项目(2022A05038)。

语种：中文

中文关键词：牡蛎;锌结合肽;稳定性;Caco-2细胞;吸收转运

外文关键词：oyster;zinc-binding peptides;stability;Caco-2 cells;absorption and transport

中文摘要：【目的】探究香港牡蛎(Crassostrea hongkongensis)锌结合肽(ZnPE)在热环境、酸碱环境中的稳定性及跨Caco-2细胞模型的吸收转运机制,为锌补剂开发提供理论依据。【方法】以牡蛎锌螯合肽(Zn-PE)和ZnSO4为对照,以总锌、锌离子的溶解率为评价指标,在40、50、60、70和80℃以及pH值为2、4、6、8、10条件下,研究ZnPE的热稳定性和酸碱稳定性;并建立Caco-2细胞模型,研究ZnPE在跨Caco-2细胞模型的吸收率、生物利用率和转运途径;通过液质联用法研究ZnPE跨Caco-2细胞模型后的序列变化。【结果】不同温度中ZnPE与Zn-PE的总锌、锌离子溶解率无显著差异(P>0.05),两者均可保持较高的稳定性。在酸性环境(pH=2)中,ZnPE和Zn-PE中总锌溶解率均在90%以上,而锌离子有一定程度的释放,溶解率分别为22.30%、33.45%,两者稳定程度降低,但ZnPE稳定性显著高于Zn-PE(P<0.05)。在碱性环境(pH=8)中,ZnPE和Zn-PE均保持稳定。ZnPE可完整、稳定的通过Caco-2细胞模型,且其锌的吸收率为74.37%、生物利用率为76.40%,显著高于Zn-PE、ZnSO_(4)(P<0.05)。ZnPE中的锌主要以肽的形式通过细胞旁路和转胞吞作用的方式跨Caco-2细胞模型转运。转运后鉴定出8种锌结合肽,其中的5种和转运前一致,表明ZnPE在吸收转运中可保持较高的稳定性。【结论】ZnPE可在热环境、酸碱环境中及跨Caco-2细胞模型转运时保持较高的稳定性。

外文摘要：【Objective】To investigate the stability of oyster(Crassostrea hongkongensis)zinc-binding peptides(ZnPE)in thermal and acidic or alkaline environments and the mechanism of absorption and transport across the Caco-2 cell monolayers model,and the conclusions may provide theoretical basis for the development of zinc supplements.【Methods】Using oyster zinc-chelating peptides(Zn-PE)and ZnSO4 as control,the stability of ZnPE was evaluated by the solubility indexes of total Zn and Zn ions at 40,50,60,70 and 80℃,and at pH values of 2,4,6,8,and 10;a Caco-2 cell monolayer model was established to study the absorption rate,bioavailability,and transport pathway of ZnPE across Caco-2 cell monolayer model.The sequence changes of ZnPE across Caco-2 cell monolayers were investigated by liquid chromatography-mass spectrometry(LC-MS/MS).【Results】Under different temperatures,there was no significant difference in the solubility rates of total zinc and zinc ions in ZnPE and Zn-PE(P>0.05),and both of them could maintain a high stability.In acidic environment(pH=2),the solubility rate of total zinc in both ZnPE and Zn-PE was above 90%,while there was a slight release of zinc ions with the solubility rate of 22.30%and 33.45%,respectively,and the stability of both of them was reduced but the stability of ZnPE was significantly higher than that of Zn-PE(P<0.05).While in alkaline environment(pH=8),both ZnPE and Zn-PE remained stable.The ZnPE could completely and stably pass through Caco-2 cells monolayers model,and its zinc absorption was 74.37%and bioavailability was 76.40%,which were significantly higher than that of Zn-PE and ZnSO_(4)(P<0.05).The zinc in the ZnPE was transported across the Caco-2 cell monolayers model mainly in the form of peptides through cell bypass by means of transcytosis.Eight zinc-binding peptides were identified after transport,five of which were consistent with those before transport,suggesting that ZnPE can maintain high stability in absorption and transport.【Conclusion】ZnPE can maintain high stability in thermal and acrid/alkaline environments and during transport across the Caco-2 cell monolayers model.