文献类型：期刊文献

英文题名：Alkaline hydrogen peroxide pretreatment combined with bio-additives to boost high-solids enzymatic hydrolysis of sugarcane bagasse for succinic acid processing

作者：Zhang, Jun[1,5];Li, Kuntai[1,5];Liu, Shucheng[1,5];Huang, Shushi[4];Xu, Chao[2,3]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol,Key Lab Adv Proc Aquat Pr, Guangdong Prov Key Lab Aquat Product Proc & Safet, Guangdong Prov Engn Technol Res Ctr Seafood,Guang, Zhanjiang 524088, Peoples R China;[2]Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Peoples R China;[3]Chinese Acad Agr Sci, Ctr Southern Econ Crops, Changsha 410205, Peoples R China;[4]Guangxi Acad Sci, Guangxi Key Lab Marine Nat Prod & Combinatorial B, Nanning 530007, Peoples R China;[5]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China

年份：2022

卷号：345

外文期刊名：BIORESOURCE TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000743263100001)、、EI(收录号：20215111354783)、Scopus(收录号：2-s2.0-85121243719)、WOS

基金：This research was financially supported by the Agricultural Science and Technology Innovation Program of China (CAAS-ASTIP-2021-IBFC) , the China Agricultural Research System for Bast and Leaf Fiber Crops (CARS-16) , the Program for Scientific Research Start-up Funds of Guangdong Ocean University (060302042006) , the Innovative Team Program of High Education of Guangdong Province (2021KCXTD021) and the Guangdong Innovation Team of Seafood Green Processing Technology (2019KCXTD011) .

语种：英文

外文关键词：Lignocellulosic biomass; Succinic acid; Pretreatment; Enzymatic hydrolysis; Fermentation

外文摘要：Alkaline hydrogen peroxide (AHP) pretreatment of sugarcane bagasse (SCB) at mild conditions was optimized with response surface methodology (RSM), then enzymatic hydrolysis was performed at high-solids substrate loading (30 %, w/v), followed by fed-batch fermentation to convert the fermentable sugars into succinic acid (SA). Results showed the AHP pretreatment conditions of H2O2 concentration 5.5 % (v/v), solid-to-liquid ratio 0.08, pretreatment temperature 65 degrees C and time 5 h could achieve the highest sugar yield (74.3 %); both additives and fed-batch strategy were favored to boost enzymatic hydrolysis, the concentration and yield of total sugars reached to 195 g/L and 70 % with cellulase dosage of only 6 FPU/g dry biomass (DM); all glucose and xylose could be utilized after fed-batch fermentation, and the obtained concentration and yield of SA reached 41.4 g/L and 63.8 %. In summary, a SA conversion rate high to 0.29 g/g SCB raw material could be achieved via the developed process.