文献类型：期刊文献

英文题名：Integrated multi-omics to analyze the generation mechanism of volatile substances in refrigerated tilapia fillets

作者：Liu, Zhenyang[1,2,3]; Xiao, Naiyong[1]; Qin, Lei[4]; Wang, Zefu[1]; Sun, Qinxiu[1]; Zhang, Di[1]; Cao, Hui[1,2,3]; Xiao, Jianbo[1,2,3]; Liu, Shucheng[1,2]

机构：[1] College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong, Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang, 524088, China; [2] Universidade de Vigo, Nutrition and Bromatology Group, Department of Analytical Chemistry and Food Science, Faculty of Science, Ourense, 32004, Spain; [3] Instituto de Agroecoloxía e Alimentación [IAA], Universidade de Vigo, Campus Auga, Ourense, 32004, Spain; [4] School of Food Science and Technology, Dalian Polytechnic University, Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian, 116034, China

年份：2026

卷号：231

外文期刊名：Food Research International

收录：EI(收录号：20260920177215)

语种：英文

外文关键词：Metabolism - Metabolites - Physiology - Proteomics

外文摘要：This study employed a non-targeted metabolomics to reveal the impact of refrigerated storage on tilapia fillets, and integrated DIA-based proteomics to investigate formation mechanisms of key volatile substances. A potential pathway map of key volatile substances generation was constructed. The results showed that, in tilapia fillets (d0 vs d1, d0 vs d3, d0 vs d5, and d0 vs d7), 5, 13, 25, and 36 metabolites were upregulated, whereas 23, 31, 41, and 38 metabolites were downregulated, respectively. These differential metabolites were mainly enriched in amino acid, nucleotide, and lipid metabolism. Integrated analysis revealed that differential metabolites such as adenosine and histidine, in conjunction with enzymes including adenylate kinase and choline-phosphate cytidylyltransferase, significantly influenced the formation of key volatile substances through seven metabolic pathways, including glycerophospholipid metabolism, glutathione metabolism, and purine metabolism. These findings provide molecular-level insights and a theoretical basis for flavor regulation of aquatic products during refrigerated storage. ? 2026