文献类型：期刊文献

英文题名：Combined effect of salinity and hypoxia on digestive enzymes and intestinal microbiota in the oyster Crassostrea hongkongensis

作者：Xie, Zhe[1,3];Li, Yuting[1,3];Xiong, Kai[1,3];Tu, Zhihan[4];Waiho, Khor[4];Yang, Chuangye[2];Deng, Yuewen[2];Li, Saishuai[1,3];Fang, James K. H.[5];Hu, Menghong[1,3];Dupont, Sam[6,7];Wang, Youji[1,3]

机构：[1]Shanghai Ocean Univ, Minist Sci & Technol, Int Res Ctr Marine Biosci, Shanghai 201306, Peoples R China;[2]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[3]Shanghai Ocean Univ, Coll Fisheries & Life Sci, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai 201306, Peoples R China;[4]Univ Malaysia Terengganu, Higher Inst Ctr Excellence HICoE, Inst Trop Aquaculture & Fisheries, Terengganu 21030, Malaysia;[5]Hong Kong Polytech Univ, Dept Food Sci & Nutr, Hung Hom, Hong Kong, Peoples R China;[6]Univ Gothenburg, Dept Biol & Environm Sci, SE-45178 Fiskebackskil, Sweden;[7]IAEA, Environm Labs, MC-98000 Principality Of Monaco, Monaco

年份：2023

卷号：331

外文期刊名：ENVIRONMENTAL POLLUTION

收录：SCI-EXPANDED(收录号：WOS:001202242800001)、、EI(收录号：20232314197240)、Scopus(收录号：2-s2.0-85161063210)、WOS

基金：This research was supported by the grants from the National Natural Science Foundation of China (42176144, 3201101951) and the Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation (2023KA04) .

语种：英文

外文关键词：Intestinal microbiota; Digestion; Oyster; Hypoxia; Salinity

外文摘要：Anthropologic activities caused frequent eutrophication in coastal and estuarine waters, resulting in diel-cycling hypoxia. Given global climate change, extreme weather events often occur, thus salinity fluctuation frequently breaks out in these waters. This study aimed to evaluate the combined effects of salinity and hypoxia on intestinal microbiota and digestive enzymes of Crassostrea hongkongensis. Specifically, we sequenced 16 S rRNA of intestinal microbiota and measured the digestive enzymes trypsin (TRS), lipase (LPS) and amylase (AMY) in oysters exposed for 28 days to three salinities (10, 25 and 35) and two dissolved oxygen conditions, normoxia (6 mg/L) and hypoxia (6 mg/L for 12 h, 2 mg/L for 12 h). Oysters in normoxia and salinity of 25 were treated as control. After 28-day exposure, for microbial components, Fusobacteriota, Firmicutes, Bacteroidota, Proteobacteria and Actinobacteriota comprised the majority for all experimental groups. Compared with the control group, the diversity and structure of intestinal microbiota tended to change in all treated groups. The species richness in C. hongkongensis intestine also changed. It was the most significant that high salinity increased Proteobacteria proportion while low salinity and hypoxia increased Fusobacteriota but decreased Proteobacteria, respectively. Additionally, Actinobacteriota was sensitive and changed under environmental stressor (P < 0.01). The prediction results on intestinal microbiota showed that, all functions of oysters were up-regulated to distinct degrees under low/high salinity with hypoxia. According to the KEGG prediction, cellular processes were more active and energy metabolism upregulated, indicating the adaptation of C. hongkongensis to environmental change. Periodical hypoxia and low/high salinity had complex effect on the digestive enzymes, in which the activity of TRS and LPS decreased while AMY increased. High/low salinity and periodical hypoxia can change the secretion of digestive enzymes and influence intestinal microbial diversity and species richness of C. hongkongensis, deducing the chronic adverse effects on the digestive physiology in long-term exposure.