文献类型：期刊文献

英文题名：Integrated application of transcriptomics and metabolomics provides insights into unsynchronized growth in pearl oyster Pinctada fucata martensii

作者：Hao, Ruijuan[1];Du, Xiaodong[1,2];Yang, Chuangye[1];Deng, Yuewen[1,2];Zheng, Zhe[1];Wang, Qingheng[1,2]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Pearl Breeding & Proc Engn Technol Res Ctr Guangd, Zhanjiang 524088, Peoples R China

年份：2019

卷号：666

起止页码：46

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：SCI-EXPANDED(收录号：WOS:000463180700006)、、WOS

基金：This work was supported by Science and Technology Program of Guangdong Province (Grant No. 2017A030307024 and 2017A030303076); the Graduate Education Innovation Program of Guangdong Ocean University (Grant No. 201828); Department of Education of Guangdong Province (Grant No. 2017KCXTD016); China Agriculture Research System (Grant No. CARS-049). Our data analysis was assisted by Biotree Biotech Co., Ltd. (Shanghai, China).

语种：英文

外文关键词：Unsynchronized growth; Metabolomics; Transcriptomic; Pinctada fucata martensii

外文摘要：Similar to other marine bivalves, Pint-matt furata martensii presents unsynchronized growth, which is one of the problems farmers currently face. However, the underlying mechanisms have not been studied. In the present study, pearl oyster P. f. martensii from cultured stocks were selected to produce a progeny stock. At 180 (lays, the stock was sorted by size, and fast- and slow-growing individuals were separately sampled. Then, metabolomic and transcriptomic approaches were applied to assess the metabolic and transcript changes between the fast- and slow-growing P.f. martensii groups and understand the mechanism underlying their unsynchronized growth. In the metabolomics assay, 30 metabolites were considered significantly different metabolites (SDMs) between the fast- and slow-growing groups and pathway analysis indicated that these SDMs were involved in 20 pathways, including glutathione metabolism; sulfur metabolism; valine, leucine, and isoleucine biosynthesis; and tryptophan metabolism. The transcriptome analysis of different growth groups showed 168 differentially expressed genes (DEGs) and pathway enrichment analysis indicated that DEGs were involved in extracellular matrix-receptor interaction, pentose phosphate pathway, aromatic compound degradation. Integrated transcriptome and metabolome analyses showed that fast-growing individuals exhibited higher biomineralization activity than the slow-growing group, which consumed more energy than the fast-growing group in response lo environmental stress. Fast-growing group also exhibited higher digestion, anabolic ability, and osmotic regulation ability than the slow-growing group. This study is the first work involving the integrated metabolomic and transcriplomic analyses lo identify the key pathways to understand the molecular and metabolic mechanisms underlying unsynchronized bivalve growth. (C) 2019 Elsevier B.V. All rights reserved.