文献类型：期刊文献

英文题名：Identification of DLD, by immunoproteomic analysis and evaluation as a potential vaccine antigen against three Vibrio species in Epinephelus coioides

作者：Pang, Huanying[1,2,3];Chen, Liming[1,2,3];Hoare, Rowena[4];Huang, Yucong[1,2,3];ZaoheWu[2,3,5];Jian, Jichang[1,2,3]

机构：[1]Guangdong Ocean Univ, Coll Fishery, 40 East Jiefang Rd, Zhanjiang 524025, Guangdong, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524025, Peoples R China;[3]Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang 524025, Peoples R China;[4]Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland;[5]Zhongkai Univ Agr & Engn, Guangzhou 510225, Guangdong, Peoples R China

年份：2016

卷号：34

期号：9

起止页码：1225

外文期刊名：VACCINE

收录：SCI-EXPANDED(收录号：WOS:000371548600012)、、Scopus(收录号：2-s2.0-84958057933)、WOS

基金：This work was supported by two research grants. First, the National Natural Science Foundation of China (No. 31402344). Second, the Guangdong Natural Science Foundation (No. S2013040014562).

语种：英文

外文关键词：Vibrio alginolyticus; Vibrio harveyi; Vibrio parahaemolyticus; Cross immunogenic proteins; Immunoproteomic; DLD (dihydrolipoamide dehydrogenase)

外文摘要：Vibrio spp. represent a serious threat to the culture of Epinephelus coioides (Orange-spotted Grouper) in Southeast Asia. In this study we used two-dimensional electrophoresis (2-DE) and Western blotting to identify common immunogenic proteins of Vibrio alginolyticus, Vibrio harveyi and Vibrio parahaemolyticus. Membranes were probed with orange-spotted grouper anti-V. alginolyticus sera and accordingly 60, 58 and 48 immunogenic protein spots were detected. By matching analysis for the three Western blotting membranes, 6 cross immunogenic spots for the three Vibrio species were identified. They were Outer membrane protein W (OmpW), dihydrolipoamide dehydrogenase (DLD), succinate dehydrogenase flavoprotein subunit(SDHA), elongation factor Ts(Ts), peptide ABC transporter periplasmic peptide-binding protein and phosphoenolpyruvate carboxykinase(PEPCK). One of the proteins, DLD, was used to evaluate the cross protective function for E. coioides with a bacterial immunization and challenge method. The relative percent survival rate of E. coioides against V. alginolyticus, V. harveyi and V. parahaemolyticus was 90%, 86% and 80%, respectively. This work may provide potential cross protective vaccine candidate antigens for three Vibrio species, and DLD may be considered as an effective cross-protective immunogen against three Vibrio species. (C) 2016 Published by Elsevier Ltd.