文献类型：期刊文献

中文题名：不同投喂方式对中国明对虾幼体生长发育及抗病力的研究

英文题名：Influence of Different Feeding Schedule on Survival,Growth,Development and Immune Responses of Fenneropenaeus Larvae(Decapoda:Penaeidae)

作者：王新霞[1,2];麦康森[1];谭北平[3];戚成震[1];艾庆辉[1];张文兵[1];徐玮[1]

机构：[1]中国海洋大学教育部海水养殖重点实验室,山东青岛266003;[2]浙江大学教育部动物分子营养学重点实验室,浙江杭州310029;[3]广东海洋大学水产学院,广东湛江524000

年份：2009

期号：3

起止页码：397

中文期刊名：中国海洋大学学报：自然科学版

收录：CSTPCD、、CSCD2011_2012、北大核心2008、北大核心、CSCD

基金：国家高技术研究发展计划项目(2006AA100313)资助

语种：中文

中文关键词：中国明对虾;幼体;存活率;生长;变态指数;免疫指标

外文关键词：Fenneropenaeus chinensis; Larvae; survival; growth; development index; immune parameters

中文摘要：研究β—1，3／1，6-D-glucan及不同投喂方式对中国明对虾（Fenneropenaeus chinensis）幼体的存活、生长、发育、非特异性免疫及抗副溶血弧菌能力的影响。将B—glucan以0．25％的剂量添加到微黏合饲料中，以不同的投喂方式投喂对虾幼体，即D0（一直投喂基础饲料）；D1（-直投喂含0．25％β-葡聚糖饲料）；3D1—3130（3d投喂含0．25％β-葡聚糖的饲料-3d投喂基础饲料）；1D1—3D0（1d投喂含0．25％β-葡聚糖的饲料一3d投喂基础饲料），共4个处理，每个处理随机设置3个重复，对虾幼体养殖于可控温的半开放系统中，每个养殖桶中盛有50L盐度为30～32消毒海水，养殖密度为100尾／L。实验结果表明，在孵化后14d，投喂D1饲料组在总存活率，变态指数，体长，特定生长率方面均高于其它各组，虽然有时差异不显著。在免疫指标方面，在9和14dph分别进行了酚氧化酶（PO）、碱性磷酸酶（ALP）、超氧化物歧化酶（SOD）、酸性磷酸酶（ACP）活力的测定，结果表明，投喂D1饲料组的4个免疫指标均高于其它3组，虽然有时差异不显著。在14dph的攻毒实验结果表明，对照组的累积死亡率显著高于D1组（P〈0．05），而1D1—3130和3D1—3D0的累积死亡率则与其它各组均不显著。实验结果表明，间隔投喂方式并不适合于对虾幼体，在中国明对虾幼体饲料中使用β-葡聚糖时，建议添加量为0．25％，并采用一直投喂的方式。

外文摘要：The present study was conducted to evaluate the effectiveness of dietary β-1, 3/1, 6-D-glucan, derived from Saccharomyces cerevisiae, and different feeding schedule in modulating the survival, growth, development, non-specific immunity and the resistance to Vibrio parahaemolyticus for Chinese shrimp（Fenneropenaeus chinensis） larvae. The β-glucan was administered orally at concentrations 0.25 % of dietary dry matter, which were fed to the larvae in three different feeding schedules： D0 （permanently control diet） ; D1 （permanently 0.25% glucan-containing diet）; 3D1-3D0（three days 0.25% glucan-containing diet-three days control diet）; 1D1-3D0（one day 0.25% glucan-containing diet-three days control diet）. So there were 4 treatments each treatment was randomly assigned to triplicate tanks, and each tank was stocked larvae at the density of 100 L^-1. The results showed that survival rate, development index （DI）, body length and SGR of larvae fed glucan - 0.25 % diet were higher than those of larvae of other three groups, although not always significant. Immune parameters, such as phenoloxidase （PO）, superoxide dismutase （SOD）, acid phosphatase （ACP） and alkaline phosphatase（ALP） of larvae were examined at 9 and 14 dph. The results suggested that these immune parameters of larvae fed glucan - 0.25 % diet were higher than those of larvae of other three groups, although not always significant. A pathogenic challenge at 14 dph showed that cumulative mortality for glucan - 0.25 % diet treatment was significantly higher than glucan-free diet treatment（P 〈 0.05）, but not significant compared with other two treatments fed in different feeding schedule. Results of the present study suggested that different feeding schedule is not suitable to shrimp larvae, since the highest values for different parameters examined were always observed in larvae fed permanent glucan - 0.25 % diet, the supplementation of β-glucan to microbound diet at concentration of approximately 0.25 % and permanent feeding are recommended for the culture of shrimp larvae.