文献类型：期刊文献

英文题名：Identification and characterization of hepcidin from Nile Tilapia (Oreochromis niloticus) in response to bacterial infection and iron overload

作者：Yin, Xiaoxue[1];Chen, Nuo[1];Mu, Liangliang[1];Bai, Hao[1];Wu, Hairong[1];Qi, Weiwei[1];Huang, Yu[2];Jian, Jichang[2];Wang, Anli[1];Ye, Jianmin[1]

机构：[1]South China Normal Univ, Sch Life Sci, Inst Modern Aquaculture Sci & Engn, Guangdong Prov Key Lab Hlth & Safe Aquaculture, Guangzhou 510631, Peoples R China;[2]Guangdong Ocean Univ, Guangdong South China Sea Key Lab Aquaculture Aqu, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China

年份：2022

卷号：546

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:000730106300006)、、WOS

基金：This project was supported by National Natural Science Foundation of China (31902396, 31972818) , China Postdoctoral Science Founda-tion (2019M652942) , Open Foundation of Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Eco-nomic Animals (KFKT2019YB07, PBEA2020YB02) , Special Funds for the Cultivation of Guangdong College Students'Scientific and Techno-logical Innovation ("Climbing Program" Special Funds, pdjh2021b0138) . Provincial Training Program of Innovation and Entrepreneurship for Undergraduates (S202010574078) .

语种：英文

外文关键词：Oreochromis niloticus; Hepcidin; Pathogenic bacteria; Iron homeostasis; Inflammatory response

外文摘要：Hepcidin (Hepc), as an antimicrobial peptide, plays a vital role in regulating the iron homeostasis and participating in innate immunity. In this study, the open reading frame of Nile tilapia (Oreochromis niloticus) hepcidin mRNA (OnHepc) was amplified, and its tissue distribution and expression pattern in response to bacterial infection and iron overload were characterized. The results showed that the ORF of OnHepc is 273 bp in length and encodes a peptide with 90 amino acids. Hepcidin has a certain degree of conservation among different species. OnHepc is widely expressed in ten tissues of natural Nile tilapia and is most abundant in the liver. The expression of OnHepc was apparently upregulated in liver, spleen, intestine and gills after challenged by S. agalactiae and A. hydrophila. In addition, the OnHepc expression in MO/Mo and hepatocytes was also upregulated after challenged by the above bacteria. On the other hand, the OnHepc transcripts were upregulated in the liver, spleen, intestine and gills after challenged by 1 mu M and 10 mu M FeCl3 solutions, and the same trends were observed in the MO/Mo and hepatocytes after challenged by ferric ions. Moreover, the OnHepc expression was significantly upregulated after stimulated by (r)OnIL-6 protein, suggesting that inflammatory responses regulate the expression of OnHepc. In summary, the present study shows that OnHepc probably gets involved in against bacterial infection and regulating the iron homeostasis in O. niloticus.