文献类型：期刊文献

英文题名：Effects of fish meal replacement by low-gossypol cottonseed meal on growth performance, digestive enzyme activity, intestine histology and inflammatory gene expression of silver sillago (Sillago sihamaForsskal) (1775)

作者：Liu, Hao[1,2];Dong, Xiaohui[1,2];Tan, Beiping[1,2];Du, Tao[3];Zhang, Shuang[1,2];Yang, Yuanzhi[1,2];Chi, Shuyan[1,2];Yang, Qihui[1,2];Liu, Hongyu[1,2]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Lab Aquat Anim Nutr & Feed, Zhanjiang 524088, Peoples R China;[2]Aquat Anim Precis Nutr & High Efficiency Feed Eng, Zhanjiang, Peoples R China;[3]Guangdong Ocean Univ Marine Biol Res Base, Zhanjiang, Peoples R China

年份：2020

卷号：26

期号：5

起止页码：1724

外文期刊名：AQUACULTURE NUTRITION

收录：SCI-EXPANDED(收录号：WOS:000563617700001)、、WOS

基金：National Natural Science Foundation of China, Grant/Award Number: 31972808; Science and Technology Department of Guangdong Province, Grant/Award Number: 2015A020209170; Earmarked Fund for China Agriculture Research System, Grant/Award Number: CARS-47

语种：英文

外文关键词：digestive enzyme; inflammatory genes; intestine histology; low-gossypol cottonseed meal; plant protein; Sillago sihama

外文摘要：A 56-day feeding trial was conducted to evaluate the effects on the growth performance, digestive enzyme activity, inflammatory genes expression and intestine histology of silver sillago,Sillago sihama(Forsskal 1775), by replacing fish meal (FM) with low-gossypol cottonseed meal (LCSM). Five isonitrogenous and isolipidic diets were formulated, including R0 group (control, containing 550.0 g/kg FM), R16 group (88.5 g/kg LCSM and 461.5 g/kg FM), R32 group (177.0 g/kg LCSM and 373.0 g/kg FM), R48 group (265.5 g/kg LCSM and 284.5 g/kg FM) and R64 group (354.0 g/kg LCSM and 196.0 g/kg FM). Fish fed R0 and R16 groups had a significantly higher weight gain rate (WGR) and specific growth rate (SGR) than R48 and R64 groups (p < .05). In contrast to whole-body crude protein, whole-body moisture increased with the FM level of substitution (p < .05). With the increased amount of LCSM in the diet, the activity of intestinal amylase (AMS) increased significantly (p < .05), and intestinal trypsin (TRP) decreased (p < .05). Dietary LCSM substitution upregulated the expression of intestinal tumour necrosis factor-alpha (TNF-alpha), the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B), and interleukin one beta (IL-1 beta), but downregulated tight junction proteins ZO-1(ZO-1), transforming growth factor beta-3 (TGF-beta 3) and interleukin 10 (IL-10) expression. Histological analysis revealed progressive morphological damage to the mid-intestine with higher levels of FM replacement. These results showed that 88.5 g/kg (16%) of FM replaced by LCSM with amino acids (methionine and lysine) supplementation did not significantly reduce growth compared with FM-based control.