文献类型：期刊文献

英文题名：Identification and characterization of tumor necrosis factor receptor (TNFR)-associated factor 3 from humphead snapper, Lutjanus sanguineus

作者：Cai, Jia[1,2,3];Xia, Hongli[1,2,3];Huang, Yucong[1,2,3];Tang, Jufen[1,2,3];Jian, Jichang[1,2,3];Wu, Zaohe[2,3,4];Lu, Yishan[1,2,3]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Guangdong, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China;[3]Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang 524088, Peoples R China;[4]Zhongkai Univ Agr & Engn, Guangzhou 510225, Guangdong, Peoples R China

年份：2015

卷号：46

期号：2

起止页码：243

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000363071200010)、、Scopus(收录号：2-s2.0-84937786151)、WOS

基金：The authors wish to thank all the laboratory members' suggestion on this manuscript. This work was supported by National Natural Science Foundation of China (U1401211, 31402335) and Educational Commission of Guangdong Province (2014GKXM046, 2013gjhz0008).

语种：英文

外文关键词：Humphead snapper; TRAF3; Immune response; Over-expression

外文摘要：Tumor necrosis factor receptor (TNFR)-associated factor 3(TRAF3) is a key regulator in TNFR and Toll-like receptor (TLRs)/RIG-I-like receptors (RLRs) signal pathway. Here, a TRAF3 gene (Ls-TRAF3, GenBank Accession No: Kj789921) is cloned from humphead snapper (Lutjanus sanguineus). The Ls-TRAF3 cDNA contains an open reading frame of 1788 bp, which encodes a polypeptide of 595 amino acids. The deduced amino acid of Ls-TRAF3 possesses a RING finger, two TRAF-type zinc fingers, a coiled-coil and a MATH domain. Ls-TRAF3 protein shares high identities with other known TRAF3 proteins. In healthy fish, Ls-TRAF3 transcripts were broadly expressed in all examined tissues with highest expression levels in spleen, liver and head kidney. Quantitative real-time PCR (qRT-PCR) analysis revealed that Ls-TRAF3 could be induced by bacteria or viral PAMP poly I:C stimulation in vivo. Here, we also showed Ls-TRAF3 that, positively regulated IRF3 and Mx upon poly I:C stimuli, whereas prevented production of proinflainmatory cytokine IL-6 after LPS injection. Moreover, over-expression of wide type (WT) Ls-TRAF3 and truncated forms, including Delta Zinc finger 1, Delta Zinc finger 2 and Delta coiled-coil suppressed NF-kappa B activity significantly, whereas the inhibitory effect of NF-kappa B was partially impaired when the RING finger or MATH domain deletion, suggesting the latter was more important for downstream signal transduction. Taken together, these results implicated that Ls-TRAF3 might play regulatory roles in immune response to pathogen invasion. (C) 2015 Elsevier Ltd. All rights reserved.