文献类型：期刊文献

中文题名：凡纳滨对虾肝胰腺组织化学过程参数的优化

英文题名：Optimization of Procedure Parameters for Hepatopancreas of Litopenaeus vanname

作者：徐德峰[1];田蒙蒙[1];李彩虹[2];孙力军[1];王雅玲[1];叶日英[1];廖建萌[3]

机构：[1]广东省水产品加工与安全重点实验室广东海洋大学食品科技学院,广东湛江524088;[2]广东医学院生物化学与分子生物学教研室,广东东莞523808;[3]国家海产品质量监督检验中心(湛江),广东湛江524000

年份：2017

卷号：17

期号：10

起止页码：122

中文期刊名：中国食品学报

外文期刊名：Journal of Chinese Institute Of Food Science and Technology

收录：CSTPCD、、北大核心2014、EI(收录号：20181304948759)、Scopus(收录号：2-s2.0-85044311610)、CSCD2017_2018、北大核心、CSCD

基金：国家自然科学基金项目(31201309;31371746;31171634;31371777);广东省科技厅项目(2013B021100016);浙江省食品科学与工程重之重一级学科开放课题(JYTsp20141042);农业部水产品加工重点实验室开放课题(NYJG201404);广东省高等学校创新强校项目(2014KTSCX078;GDOU2013050205;GDOU2013050203)

语种：中文

中文关键词：凡纳滨对虾;肝胰腺;石蜡切片;HE染色

外文关键词：Litopenaeus vanname; hepatopancreas; paraffin section; hematine-eosin staining

中文摘要：鉴于肝胰腺组织在凡纳滨对虾生长和免疫方面的重要生理意义,以及与对虾冷藏期间品质劣变的密切相关性,本研究通过比较不同条件下石蜡切片对凡纳滨对虾肝胰腺组织HE染色结果的影响,确定其组织化学适宜参数。凡纳滨对虾肝胰腺用Carnoy固定液在室温下固定12 h,用70%,80%,95%及无水乙醇Ⅰ各脱水30min,再继续用无水乙醇Ⅱ脱水40 min,二甲苯40 min透明2次,充分浸蜡后切片(8μm),将切片经苏木精染色40 min,盐酸乙醇分化1 s,伊红复染5 min。显微观察结果显示:细胞染色均匀,细胞核呈蓝紫色,细胞质呈紫红色,背景清晰。

外文摘要：Although the hepatopancreas tissue plays a vital physiological significance in growth and immunity of Litopenaeus vanname and it has a close relationship with the quality deterioration of shrimp during cold storage,the corresponding researches have been scarcely reported so far.The influence of paraffin-embedded section and staining parameters on the visualization of sections were thus investigated to seek the appropriate tissue preparation and processing.The results showed after fixation in Carnoy solution for 12 h and dehydration with the serial concentration of ethanol such as 70%,80%,95%,and 100% for 30 min respectively,the tissue was transpired twice with xylene for 40 min.With a sufficient immersing and embedding in paraffin wax,subsequent sectioning is performed on the Leica slicer with 8 μm in thickness and the obtained sections were immediately stained with hematine for 40 min.After differentiation in ethanol acidized with hydrochloric acid for 1 second,counterstaining of section with eosin for 5 min was conducted.Under these parameters the morphology of hepatopancreas sections observed with microscopy was clear in which the nuclear manifested bluish violet and sharp red was observed in cytoplasm parts.